江苏大学学报（医学版）2026，Vol.36Issue(1)：30-35,43,7.DOI:10.13312/j.issn.1671-7783.y240180
胃癌三级淋巴结构ARHGAP12表达分析
Analysis of ARHGAP12 expression in tertiary lymphoid structures of gastric cancer
摘要
Abstract
Objective:To investigate the expression levels of Rho GTPase-activating protein 12(ARHGAP12)within tertiary lymphoid structures(TLS)in gastric cancer and its impact on tumor immune response.Methods:ARHGAP12 mRNA expression differences between gastric cancer(n=414)and normal tissues(n=211)were analyzed using TCGA databases.Western blotting was employed to validate the protein expression levels of ARHGAP12 in 12 pairs of gastric cancer and adjacent tissue samples.Immunofluorescence was used to detect ARHGAP12 protein expression in TLS and tumor-draining lymph nodes(TDLN)in gastric tissue pathological sections.Hallmark and KEGG pathway enrichment analyses were conducted to assess the correlation between ARHGAP12 mRNA expression and the activation of immune-related pathways.Immunohistochemical staining was performed to examine the protein expression levels of ARHGAP12 in pathological sections of tumor tissues from 28 gastric cancer patients,and clinical data were analyzed to explore the correlation between cytokine expression levels and ARHGAP12 immunohistochemical staining scores.KM plotter database was used to analyze the relationship between ARHGAP12 mRNA expression and prognosis of patients with gastric cancer.Results:TCGA database analysis showed that ARHGAP12 mRNA expression in gastric cancer tissues was significantly higher than that in adjacent tissues(P＜0.001).Western blotting results indicated that there was no significant difference in ARHGAP12 protein expression levels between the gastric adenocarcinoma and adjacent tissue samples(P＞0.05).Multiplex immunofluorescence results demonstrated higher expression of ARHGAP12 protein in gastric cancer cells,TLS,and TDLN regions(P＜0.05).Hallmark and KEGG pathway enrichment analysis suggested that ARHGAP12 mRNA expression is involved in the activation of interferon response pathways and antigen presentation processes.Furthermore,high ARHGAP12 immunohistochemical staining score was positively correlated with preoperative serum IFN-γlevels in patients with gastric cancer(P＜0.05).KM plotter database analysis showed that high expression of ARHGAP12 mRNA in gastric cancer tissues is positively correlated with longer overall survival period(P＜0.05).Conclusion;ARHGAP12 is highly expressed in gastric cancer TLS,and it is positively correlated with the activation of interferon-γ-related anti-tumor immune pathways.关键词
胃癌/Rho GTP酶激活蛋白12(ARHGAP12)/三级淋巴结构/干扰素反应/肿瘤引流淋巴结Key words
gastric cancer/Rho GTPase-activating protein 12(ARHGAP12)/tertiary lymphatic structures/interferon response/tumor-draining lymph nodes分类
医药卫生引用本文复制引用
季湧,朱伟,高峰,陈诗涵,徐晓凤,吕剑,龙聪,蔡圣强,徐传奇,陈静,朱淼琳..胃癌三级淋巴结构ARHGAP12表达分析[J].江苏大学学报（医学版）,2026,36(1):30-35,43,7.基金项目
江苏省卫生健康委员会医学科研项目(No.X373) （No.X373）
