江苏大学学报（医学版）2026，Vol.36Issue(1)：44-50,7.DOI:10.13312/j.issn.1671-7783.y240098
藏红花素通过调控Smad依赖性信号通路抑制甲状腺未分化癌细胞上皮-间质转化
Crocin inhibits epithelial-mesenchymal transition in anaplastic thyroid carcinoma cells by regulating the Smad-dependent signaling pathway
摘要
Abstract
Objective:To explore the effect of crocin on the migration and invasion ability of anaplastic thyroid carcinoma(ATC)cells and its molecular mechanism of regulating epithelial-mesenchymal transition(EMT)via the Smad-dependent signaling pathway.Methods:ATC cells were treated with different concentrations of crocin.Cell proliferation and apoptosis were evaluated by CCK-8 assay and flow cytometry,respectively.The effects of crocin on ATC cell migration and invasion were detected by Transwell assays.Western blotting analysis was used to examine the expression of EMT markers(E-cadherin,N-cadherin,vimentin,fibronectin)and Smad signaling pathway-related proteins.A subcutaneous tumor model was established using BHT-101 cells to evaluate the inhibitory effect of crocin on tumor invasiveness.Immunohistochemistry and Western blotting were performed to detect the expression changes of MMP-2,MMP-9,N-cadherin and vimentin in tumor tissues.Results:At concentrations below 40 μmol/L,crocin treatment significantly reduced cell invasion and migration compared with the untreated group(P＜0.01),accompanied by increased E-cadherin expression and decreased N-cadherin,vimentin and fibronectin expression.The inhibitory effect of crocin on Smad2/3 phosphorylation was alleviated after exogenous TGF-β intervention.In vivo experiments showed that crocin notably downregulated the expression of MMP-2,MMP-9,N-cadherin and vimentin in BHT-101 subcutaneous xenograft tumors.Conclusion:Crocin can inhibit the invasion and EMT of ATC cells by modulating the Smad-dependent signaling pathway.关键词
甲状腺未分化癌/迁移/侵袭/Smad信号通路/藏红花素Key words
anaplastic thyroid carcinoma(ATC)/migration/invasion/Smad-dependent signaling/crocin分类
医药卫生引用本文复制引用
吴丹,高云,潘兰芬,石磊,李芳,徐松,邓志勇..藏红花素通过调控Smad依赖性信号通路抑制甲状腺未分化癌细胞上皮-间质转化[J].江苏大学学报（医学版）,2026,36(1):44-50,7.基金项目
国家自然科学基金资助项目(82103288) （82103288）
昆山市级科技专项(KS2204) （KS2204）
