现代畜牧兽医Issue(1)：25-29,5.DOI:10.20154/j.cnki.issn1672-9692.2026.01.006
3种猪繁殖与呼吸综合征病毒抗体ELISA试剂盒的比较
Comparison of three ELISA kits for detecting antibodies against porcine reproductive and respiratory syndrome virus
摘要
Abstract
This study aimed to evaluate the detection performance of three commercial porcine reproductive and respiratory syndrome virus(PRRSV)antibody ELISA kits.Three negative serum samples,three positive serum samples,and 409 clinical serum samples were selected to compare the analytical sensitivity,intra-batch repeatability,and negative and positive agreement rates of the three kits.The results showed that the analytical sensitivity of Kit C is the highest,and Kits A and B are basically the same.The intra-batch coefficient of variation for Kits A,B,and C was less than 4%,indicating good repeatability across all kits.Among them,Kit B demonstrated the best repeatability,followed by Kit C.The positive agreement rates for Kits B and C were both 100.00%,while that for Kit A was 98.08%.The diagnostic sensitivity of the three kits ranked as B=C＞A.The negative agreement rate for Kit A was 100.00%,whereas those for Kits B and C were 99.59%and 97.22%,respectively.The diagnostic specificity ranked as A＞B＞C.The study indicates that Kit B achieved the highest overall performance evaluation,with excellent repeatability,high diagnostic sensitivity,and high diagnostic specificity,making it the preferred choice for antibody detection.If clinical testing prioritizes sensitivity,Kit C,which has higher analytical sensitivity and diagnostic sensitivity,may be selected.If clinical testing emphasizes specificity,Kit A,with higher diagnostic specificity,is recommended.关键词
PRRSV/ELISA/抗体检测/检测性能Key words
PRRSV/ELISA/Antibody detection/Detection performance分类
农业科技引用本文复制引用
田原,曹静,柴文娴,余昊天,于重伟,夏福芳..3种猪繁殖与呼吸综合征病毒抗体ELISA试剂盒的比较[J].现代畜牧兽医,2026,(1):25-29,5.基金项目
常州市农业科技创新和示范推广项目[KCSF(2024)02] （2024）
