内科理论与实践2025，Vol.20Issue(6)：449-456,8.DOI:10.16138/j.1673-6087.2025.06.04
SOX9通过抑制miR-204/-211促进类风湿关节炎成纤维样滑膜细胞增殖
SOX9 promotes proliferation of fibroblast-like synoviocytes in rheumatoid arthritis by inhibiting miR-204/-211
摘要
Abstract
Objective To investigate the effect of sex determining region Y-box transcription factor 9(SOX9)on the function of fibroblast-like synoviocyte(FLS)in rheumatoid arthritis(RA)by inhibiting micro-ribonucleic acid(miRNA)-204 and-211,and to explore the underlying mechanism.Methods A collagene-induced arthritis(CIA)model was established by type Ⅱ collagen induction.FLS were isolated from the knee joint synovial tissue of CIA mice by digestion with type Ⅳ collagenase.The expression level of Sox9 in FLS was detected by quantitative real-time polymerase chain reaction(qPCR).Small interfering ribonucleic acid(siRNA)was used to inhibit Sox9 expression,and cell proliferation was assessed by CCK-8 assay and 5-ethynyl-2'-deoxyuridine(EdU)staining.Cell senescence was analyzed by senescence-associated β-galactosidase(SA-β-gal)staining,and the expression of cyclin-dependent kinase inhibitor(CKI)p16,p21,p53 and miR-204,miR-211 was detected by qPCR.The HDOCK online server(http://hdock.phys.hust.edu.cn/)was used to analyze the binding potential between miR-204/miR-211 and SOX9.Compared with FLS from normal mice,the messenger ribonucleic acid(mRNA)level of SOX9 was significantly increased in CIA-FLS.Knock down of Sox9 can promote cell senescence,thereby inhibiting FLS proliferation.Both miR-204 and miR-211 could stably bind to SOX9.Knockdown of Sox9 in CIA-FLS can increase the expression of miR-204/-211.Conclusions SOX9 can promote the proliferation of CIA-FLS and inhibit their senescence by down-regulating the expression of miR-204 and miR-211.关键词
性别决定区Y框蛋白9/miR-204/-211/成纤维样滑膜细胞Key words
SOX9/miR-204/-211/Fibroblast-like synoviocyte分类
医药卫生引用本文复制引用
杨祎蕾,刘静,范凯健,王婷玉..SOX9通过抑制miR-204/-211促进类风湿关节炎成纤维样滑膜细胞增殖[J].内科理论与实践,2025,20(6):449-456,8.基金项目
国家自然科学基金项目(81874011、82172383) （81874011、82172383）
