内科理论与实践2025，Vol.20Issue(6)：457-461,5.DOI:10.16138/j.1673-6087.2025.06.05
紫花前胡素在动脉粥样硬化中的抗炎作用及其药物安全性评估
Anti-inflammatory effects and safety evaluation of decursin in atherosclerosis
摘要
Abstract
Objective To investigate the anti-inflammatory effects and safety of decursin in atherosclerosis,and to verify its regulatory role in macrophage inflammatory responses.Methods An atherosclerosis model was established using apolipoprotein E deficient(ApoE-/-)male mice fed a high-fat diet.RAW 264.7 macrophages were stimulated with oxidized low-density lipoprotein(oxLDL)to induce foam cell formation.Oil Red O(ORO)staining was employed to measure the reduction in aortic plaque area.Western blotting was performed to detect the phosphorylation levels of nuclear factor-κB(NF-κB).Inflammatory cytokine levels in serum and cell supernatants were analyzed using inflammatory cytokine arrays.Systemic toxicity of decursin was assessed by hematoxylin-eosin(HE)staining.Results Decursin significantly reduced aortic plaque area and inhibited the excessive activation of NF-κB in oxLDL-stimulated macrophages.It downregulated the expression of pro-inflammatory cytokines[interleukin(IL)-2 and interferon(IFN)-γ]while upregulating anti-inflammatory cytokines(IL-4 and IL-10).Furthermore,in the ApoE-/-mouse model,decursin similarly significantly lowered serum levels of pro-inflammatory cytokines and elevated those of anti-inflammatory cytokines.HE staining results showed that decursin caused no obvious pathological damage to major organs.Conclusions Decursin exerts significant anti-inflammatory effects by regulating macrophage inflammatory responses,alleviates atherosclerotic lesions,and demonstrates a high safety profile,suggesting its potential as a therapeutic agent for atherosclerosis.关键词
动脉粥样硬化/巨噬细胞/炎症/核因子κB/紫花前胡素Key words
Atherosclerosis/Macrophage/Inflammation/Nuclear factor-κB/Decursin分类
医药卫生引用本文复制引用
陈辉,杨玲,赵安琪,查晴,杨克,刘艳..紫花前胡素在动脉粥样硬化中的抗炎作用及其药物安全性评估[J].内科理论与实践,2025,20(6):457-461,5.基金项目
国家自然科学基金项目(82070401) （82070401）
