中国海洋大学学报（自然科学版）2026，Vol.56Issue(2)：37-44,8.DOI:10.16441/j.cnki.hdxb.20240354
基于细胞松弛素B和低渗条件的异源四倍体牡蛎诱导方法比较研究
A Comparative Study on the Tetraploid Induction Methods of Allotetraploid Oyster by Cytochalasin B and Hypotonic Treatment
摘要
Abstract
To investigate the tetraploid induction effect between two allotetraploid oysters(GGAG—GGA ♀×GG ♂ and AAGG—AAG ♀×GG ♂)under varying cytochalasin B(CB)concentration(0.2,0.4,0.6,0.8 mg/L),salinity gradient(6,8,10,12)and induction duration(10,15,20,25 min),we analyzed the cleavage rate,hatching rate,tetraploidy rate and induction efficiency.At the same time,the growth and survival of the early larvae were analyzed.The results showed that the optimal method of inducing tetraploid of GGAG was 0.6 mg/L of CB and 15 min.The tetraploidy rate of(42.75±2.89)%was obtained with the method.The salinity 8 and 15 min was also the optimal method,with which the tetraploidy rate of(32.28±1.97)%was obtained.For AAGG,0.6 mg/L of CB and 20 min was optimal,with which the tetraploidy rate of(47.03±2.66)%was obtained.The salinity 10 and 15 min was the optimal method,with which the tetraploidy rate of(33.06±1.18)%was obtained.关键词
异源四倍体牡蛎/多倍体诱导/细胞松弛素B/低渗Key words
allotetraploid oyster/polyploid induction/cytochalasin B/hypotonic treatment分类
农业科技引用本文复制引用
毕文龙,徐成勋,李琪..基于细胞松弛素B和低渗条件的异源四倍体牡蛎诱导方法比较研究[J].中国海洋大学学报（自然科学版）,2026,56(2):37-44,8.基金项目
山东省农业良种工程项目(2022LZGCQY010) （2022LZGCQY010）
福建省科技计划项目(2024N3003)资助Supported by the Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province(2022LZGCQY010) （2024N3003）
the Science and Technology Project of Fujian Province(2024N3003) （2024N3003）
