中国海洋大学学报（自然科学版）2026，Vol.56Issue(2)：45-51,7.DOI:10.16441/j.cnki.hdxb.20250005
细胞松弛素B和低渗诱导岩牡蛎三倍体效果评价
Efficacy Assessment of Cytochalasin B and Hypotonic Treatment in Triploid Induction of Crassostrea nippona
摘要
Abstract
To explore the optimal conditions for inducing triploid oyster(Crassostrea nippona),the effects of different induction methods(cytochalasin B and hypotonic treatment),induction durations,and induction times on cleavage rate,hatching rate,triploid rate and comprehensive evaluation index were examined in this study.Additionally,the impact of the two induction methods on larval growth and survival was analyzed.When cytochalasin B(CB)concentration was 0.5 mg/L,the induction duration was 16 min,and the induction was initiated when 30%of the first polar body(PB1)was released,the triploid rate reached(69.71±2.59)%at which the comprehensive evaluation index was the highest(42.39%).For the hypotonic treatment,at salinity 7,with the same induction duration and time(30%PB1 release),the triploid rate was(41.82±1.79)%with a comprehensive evaluation index of 24.82%.Regarding survival rate,both CB and hypotonic groups exhibited significantly lower survival rates than the control.The survival rate of the hypotonic group was higher than that of the CB group within the first 6 days but dropped below it after 8 days.In summary,the optimal condition for inducing triploid C.nippona is 0.5 mg/L CB concentration,initiating induction at 30%PB1 release,and maintaining treatment for 16 min.With the optimized method,the highest triploid rate,survival rate and comprehensive evaluation index were observed.关键词
岩牡蛎/三倍体/细胞松弛素B/低渗Key words
Crassostrea nippona/triploid/cytochalasin B/hypotonic treatment分类
农业科技引用本文复制引用
王子恒,程庚,周建民,徐成勋,李琪..细胞松弛素B和低渗诱导岩牡蛎三倍体效果评价[J].中国海洋大学学报（自然科学版）,2026,56(2):45-51,7.基金项目
山东省农业良种工程项目(2022LZGCQY010) （2022LZGCQY010）
福建省科技计划项目(2024N3003)资助Supported by the Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province(2022LZGCQY010) （2024N3003）
the Science and Technology Project of Fujian Province(2024N3003) （2024N3003）
