中国海洋大学学报（自然科学版）2026，Vol.56Issue(2)：60-70,11.DOI:10.16441/j.cnki.hdxb.20240313
马里亚纳海沟挑战者深渊沉积物异养细菌的分离培养及分类鉴定
Isolation and Taxonomic Identification of Cultivable Heterotrophic Bacteria from Deep Sediment of the Mariana Trench
摘要
Abstract
To investigate the diversity of culturable heterotrophic bacteria in deep-sea sediments from the Mariana Trench,this study focused on a sediment core collected from the Challenger Deep at a depth of 10 816 meters on July,2020.Samples from the surface and various depths within the sediment core were selected,and multiple enrichment and isolation techniques were employed to enhance the cultur-ability of marine bacteria from extreme environments.Cultivation was performed at three temperatures(4,16,and 28 ℃)using two solid media(MA and R2A)for direct plating,and three enrichment media(IMB,IMB-DMSP,and IMB-Rpf)for selective cultivation.A total of 517 strains of heterotrophic bac-teria were obtained,and preliminary identification of potentially novel bacterial taxa was conducted based on 16S rRNA gene sequence comparisons.The results revealed that the isolated 517 strains be-longed to 4 phyla(Proteobacteria,Firmicutes,Actinobacteria,and Bacteroidetes),8 classes,30 or-ders,42 families,74 genera,and 144 species.Different enrichment strategies yielded distinct bacterial isolates,with the IMB-Rpf medium enabling the recovery of a broader diversity of microbial species.The addition of revival factors such as DMSP and supernatants from Mcrococcus luteus had selective en-richment effects on various culturable microbial populations in the sediments.Additionally,composi-tional differences in the cultured bacterial species were observed among different sediments depths.51 strains from the 4 phyla represented potential new taxonomic units,including 3 potential new genera and 41 potential new species.Notably,2 strains of them belonged to the class Sphingobacteriia,which have not previously been isolated from Mariana Trench sediments.The research results will provide a substantial resource of deep-sea bacterial strains for future scientific studies and offer new insights into the isolation and cultivation of microorganisms from extreme deep-sea environments.关键词
马里亚纳海沟/深部沉积物/异养细菌/富集培养/分离鉴定Key words
Mariana Trench/deep sediment/heterotrophic bacteria/enrichment culture/isolation and identification分类
生物科学引用本文复制引用
黄蓉,刘荣华,刘吉文,张晓华..马里亚纳海沟挑战者深渊沉积物异养细菌的分离培养及分类鉴定[J].中国海洋大学学报（自然科学版）,2026,56(2):60-70,11.基金项目
国家自然科学基金项目(92251303) （92251303）
山东省自然科学基金项目(ZR2024JQ006)资助Supported by the National Natural Science Foundation of China(92251303) （ZR2024JQ006）
the Natural Science Foundation of Shandong Province(ZR2024JQ006) （ZR2024JQ006）
