热带作物学报2026，Vol.47Issue(1)：17-31,15.DOI:10.3969/j.issn.1000-2561.2026.01.003
甘蔗GLY基因家族的鉴定及GLYI-1基因的功能分析
Genome-wide Identification of the GLY Gene Family and Functional Characterization of the GLYI-1 Gene in Sugarcane
摘要
Abstract
The glyoxalase system,comprising glyoxalase I(GLYI)and glyoxalase II(GLYⅡ),serves as a key mecha-nism for detoxifying excess methylglyoxal(MG)in plants and plays an important role in responses to abiotic stress and pathogen infection.To date,genome-wide identification and expression analysis of GLY gene family have not been re-ported in sugarcane.In this study,we identified 50 SsGLYI genes and 52 SsGLYⅡ genes from the wild sugarcane Sac-charum spontaneum,and systematically analyzed the physicochemical properties,gene structures,promoter cis-acting elements,and expression patterns.SsGLY genes were randomly distributed across 30 chromosomes.All 43 SsGLYI members contained a conserved Motif1(PF00903),while all SsGLYII proteins possessed a conserved Motif1(PF00753).A total of 39 SsGLYI proteins contained four metal-binding sites(H/Q,L,H,E),except SsGLYII8.1-8.4 from subfamily IV,which were Zn2+-dependent,the remaining SsGLYI proteins were Ni2+-dependent.Only 26 SsGLYI proteins con-tained both the THxHxDH metal-binding domain and the C/GHT active site.Collinearity analysis identified 41 pairs of collinear genes,among which 39 pairs had Ka/Ks values less than 1,indicating that the SsGLY gene family has under-gone predominant purifying selection during evolution.The expression levels of SsGLYI subfamily V and SsGLYⅡsubfamily Ⅲ were higher than those of the other four subfamilies under infection by Sporisorium scitamineum.Notably,the expression of SsGLYI3.4 showed differences in its expression patterns between the resistant genotype YT93-159 and the susceptible genotype ROC22.Furthermore,we cloned the homologous gene ScGLYI-1 of SsGLYI3.4 from YT93-159,which was constitutively expressed and primarily localized to the chloroplast.Meanwhile,the expression of ScGLYI-1 could be up-regulated by exogenous salicylic acid(SA)and smut pathogen stress,indicating that this gene may play a crucial role in response to pathogen stress in sugarcane.This study would provide a theoretical foundation for further elucidating the function of glyoxalase genes in sugarcane.关键词
甘蔗/乙二醛酶基因家族/生物信息学/甘蔗黑穗病/表达模式Key words
sugarcane/glyoxalase gene family/bioinformatics/sugarcane smut/expression pattern分类
农业科技引用本文复制引用
孙欣路,赵婉莹,林佩霞,崔世江,李振香,王东姣,张媛媛,曾真,阙友雄,吴期滨..甘蔗GLY基因家族的鉴定及GLYI-1基因的功能分析[J].热带作物学报,2026,47(1):17-31,15.基金项目
热带作物生物育种全国重点实验室创新团队项目(No.NKLTCBCXTD24,No.NKLTCBCXTD38) （No.NKLTCBCXTD24,No.NKLTCBCXTD38）
中国热带农业科学院国家热带农业科学中心科技创新团队项目(No.CATASCXTD202402). （No.CATASCXTD202402）
