山东农业大学学报（自然科学版）2026，Vol.57Issue(1)：13-22,10.DOI:10.3969/j.issn.1000-2324.2026.01.002
工程碎石土环境中枯草芽孢杆菌对高羊茅生长的影响
Effect of bacillus subtilis on the Growth of Festuca arundinacea in Engineered Gravel Soil
摘要
Abstract
Festuca arundinacea,a resilient and widely adapted graminaceous plant,is widely used in ecological restoration and slope protection projects.However,its growth is limited in engineered gravel soil—a loose,infertile,and microbe-deficient product of tunnel drilling and blasting.Studies have shown that the root system of F.arundinacea provides a carbon source for microorganisms and regulates soil enzymes by secreting organic substances.Nevertheless,limitations such as the low cohesion and nutrient deficiency of engineered gravel soils still severely restrict the plant growth.Plant-microbe synergistic remediation is an effective strategy to alleviate such adverse conditions.Among them,plant growth-promoting rhizobacteria(PGPR),such as Bacillus subtilis can promote plant growth by secreting organic acids,solubilizing phosphorus and potassium,and inducing stress resistance.Meanwhile,the root exudates of F.arundinacea act as carbon sources to enhance the stability of the microbial community,forming a reciprocal relationship between the F.arundinacea and Bacillus subtilis.However,most existing studies focus on conventional soil environments,and the correlation between the two in engineered gravel soil substrate remains unclear.This experiment conducts an indoor pot experiment,selecting F.arundinacea as the plant material.It treats the plants with nutrient solution and B.subtilis separately to investigate the effects of changes in nutrients and enzyme activities of engineered gravel soil on the growth status of F.arundinacea under artificial intervention.The results show that compared with the control group,the addition of nutrient solution increases the contents of organic carbon and ammonium nitrogen,but the poor water retention capacity of engineered gravel soil leads to nutrient loss.The interaction between B.subtilis and F.arundinacea improves engineered gravel soil,significantly increasing the contents of organic carbon,available phosphorus,available potassium,and nitrate nitrogen,thereby promoting nutrient accumulation.Sucrase and urease activities increase to varying degrees in both treatment groups,promoting the biomass accumulation of F.arundinacea.The contribution of sucrase to the root-shoot ratio and belowground dry weight increases to different degrees in both the B.subtilis and nutrient treatment groups,which is significantly superior to that in the control group.In contrast,the contribution of total phosphorus and total potassium to the root-shoot ratio and belowground dry weight is significantly enhanced only in the B.subtilis treatment group.This study provides feasible measures for improving the unfavorable growth performance of F.arundinacea in engineered gravel soil.关键词
碎石土/高羊茅/根际促生菌/酶活力/土壤养分Key words
Gravel soil/Festuca arundinacea/plant growth-promoting rhizobacteria/enzyme activity/soil nutrients分类
农业科技引用本文复制引用
白金鑫,詹登海,石立豪,胡芸菲,韩洪瑞,罗正宇..工程碎石土环境中枯草芽孢杆菌对高羊茅生长的影响[J].山东农业大学学报（自然科学版）,2026,57(1):13-22,10.基金项目
西南诸河上游重大水电工程扰动区一体化边坡防护与生态修复技术(2024YFF1307800) （2024YFF1307800）
