山东农业科学2026，Vol.58Issue(1)：8-16,9.DOI:10.14083/j.issn.1001-4942.2026.01.002
甘蓝型油菜冷调节基因BnaCOR27的克隆及表达分析
Cloning and Expression Analysis of BnaCOR27 Gene in Brassica napus
摘要
Abstract
The COR27 gene is a cold regulatory gene important in plant response to cold stress.To explore the function of COR27 gene from Brassica napus,the variety of ZS11 was used and a COR27 full-length CDS se-quence of 723 bp was cloned.,It encoded 240 amino acids and was named as BnaCOR27.Bioinformatics analy-sis showed that the BnaCOR27 protein was a hydrophilic protein with poor stability,had no transmembrane do-main and signal peptide,and was a non-secreted protein.Multiple sequence alignment revealed low sequence i-dentity between BnaCOR27 and COR27 proteins of other 17 species such as Brassica rapa,Hirschfeldia incana,and Raphanus sativus.The phylogenetic evolutionary tree showed that the COR27 proteins of Cruciferae were clustered together,and BnaCOR27 had the most close evolutionary relationship with COR27 of B.rapa.Promoter element analysis showed that the expression of BnaCOR27 was not only induced by low temperature but also reg-ulated by light signals,and it played an important role in response to cold stress as well as other stress stresses.The results of real-time fluorescence quantitative PCR detection showed that BnaCOR27 had the expression pattern of leaf﹥root﹥flower﹥stem﹥silique in B.napus ZS11.The overexpression vector pCAMBIA1301-BnaCOR27 was successfully constructed.These results could lay the foundation for in-depth exploration of the mechanism of BnaCOR27 gene in cold resistant process and provide a theoretical guidance for molecular breeding of B.napus.关键词
COR27/甘蓝型油菜/冷胁迫/生物信息学分析/表达分析/过表达载体Key words
COR27/Brassica napus L./Cold stress/Bioinformatics analysis/Expression analysis/Over-expression vector分类
农业科技引用本文复制引用
彭泱瑜,陈雨蝶,张泽荣,洪波,邢曼,熊兴华..甘蓝型油菜冷调节基因BnaCOR27的克隆及表达分析[J].山东农业科学,2026,58(1):8-16,9.基金项目
"十四五"国家重点研发计划项目(2023YFD1201403) （2023YFD1201403）
国家科技创新2030 重大项目(2022ZD0400901) （2022ZD0400901）
