山东农业科学2026，Vol.58Issue(1)：36-46,11.DOI:10.14083/j.issn.1001-4942.2026.01.005
基于SSR标记的4种榧树属植物遗传多样性及遗传结构研究
Study on Genetic Diversity and Genetic Structure of Four Torreya Species Based on SSR Markers
摘要
Abstract
To unveil the genetic diversity and structure of Torreya plants and lay a theoretical foundation for evaluating,protecting and utilizing germplasm resources,a total of 295 samples from 4 Torreya species were used as materials.After selection,15 pairs of selected primers were employed for SSR-PCR amplification across all samples,and the amplification products were analyzed by capillary electrophores detection to explore their genetic diversity and structure,and cluster analysis was conducted.The results revealed the following in-sights:(1)Based on the effective allele,the genetic diversity from high to low was Torreya fargesii(P=87.50%,He=0.396,I=0.740),Torreya taxifolia(P=86.67%,He=0.342,I=0.596),Torreya parvifolia(P=73.33%,He=0.285,I=0.480),and Torreya yunnanensis(P=57.57%,He=0.194,I=0.332).(2)The average Fis value of 21 Torreya populations was 0.174,the average Fst value was 0.490,and the Nm was 0.260,indicating that gene exchange occurred infrequently.The Fit value of Torreya populations was greater than the Fis value,suggesting that the heterozygous level between populations was higher than that within pop-ulations.(3)The results of the analysis of molecular variance indicated that the genetic variation of Torreya plants mainly originated among populations with a variable coefficient of 58%.The Nei's standard genetic dis-tance among 21 populations ranged from 0.018 to 2.453 with an average of 0.609.Overall,the genetic distance was positively correlated with the geographical distance(r=0.759,P<0.05),while there was no significant correlation between the genetic distance and geographical distance in T.yunnanensis and T.fargesii.(4)Ge-netic similarity coefficients among the 21 populations were ranged from 0.403 to 0.983.Using a threshold of 0.12,the populations were grouped into 2 major categories with T.taxifolia forming one category and the re-maining 20 populations of the other three species another.At a threshold of 0.58,they could be divided into 4 major categories.关键词
榧树属/SSR标记/遗传多样性/遗传结构Key words
Torreya/SSR marker/Genetic diversity/Genetic structure分类
农业科技引用本文复制引用
马长乐,张金丽,杨建欣,李靖,岳亮亮,周炳江..基于SSR标记的4种榧树属植物遗传多样性及遗传结构研究[J].山东农业科学,2026,58(1):36-46,11.基金项目
云南省农业联合专项面上项目(202101BD070001-100) （202101BD070001-100）
云南省农业联合专项重点项目(202301BD070001-150) （202301BD070001-150）
国家自然科学基金项目(31360037) （31360037）
云南省教育厅科学研究基金项目(2021Y260,2023Y0743) （2021Y260,2023Y0743）
