山西农业科学2026，Vol.54Issue(1)：39-52,14.DOI:10.26942/j.cnki.issn.1002-2481.2026.01.05
高粱FAD基因家族的全基因组鉴定与生物信息学分析
Whole Genome Identification and Bioinformatics Analysis of FAD Gene Family in Sorghum
摘要
Abstract
Fatty acid desaturases(FADs)play an important role in plant growth,development,and stress response by catalyzing the formation of double bonds in fatty acid acyl chains to regulate unsaturated fatty acid synthesis.To analyze the molecular characteristics of the FAD gene family in sorghum,in this study,a combined screening strategy of Blastp and Hidden Markov Model(HMM)was used based on 20 FAD protein sequences in rice to identify 24 SbFAD gene family members from the sorghum genome,and bioinformatics analysis was systematically conducted.The research results showed that 24 SbFAD genes were unevenly distributed on 9 chromosomes,with the highest distribution density on chromosomes 1,2,and 4(each containing 4 members).The analysis of protein physicochemical properties showed that the number of amino acid residues in SbFAD protein ranged from 325 to 480,with a theoretical isoelectric point(pI)of 5.26-9.33,and most of them were hydrophilic.Systematic evolutionary analysis revealed that SbFAD protein was highly conserved with rice homologous genes,and members of the same subfamily had similar conserved motif compositions.The gene structure showed significant differences,with a range of 2-10 exons,among which SbFAD21 contained 10 exons.All members contained typical fatty acid desaturase domains,with SbFAD06/SbFAD09 carrying an additional cytochrome b5(Cyt-b5)domain.Subcellular localization prediction showed that it was mainly located in chloroplasts and plasma membranes,with secondary structures mainly consisting of alpha helixes and random coils.Gene replication analysis suggested that fragment duplication events were involved in family amplification and highly homologous to FADs in maize and rice.The promoter region was enriched with hormone responsive,abiotic stress,and developmental related cis acting elements.Expression profiling analysis showed that six members of Heatmap clustering Ⅰ were widely expressed throughout the entire growth period and multiple tissues of sorghum.This study provided a systematic elucidation of the evolutionary characteristics and functional differentiation patterns of the sorghum FAD gene family.关键词
高粱/脂肪酸去饱和酶/隐马尔可夫模型/基因家族/亚细胞定位Key words
sorghum/fatty acid desaturase/Hidden Markov Model/gene family/subcellular localization分类
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张晓春,王要闯,刘平丽,徐玲,王凤宇,高珂珂,王军凤,王栋,宋军锋..高粱FAD基因家族的全基因组鉴定与生物信息学分析[J].山西农业科学,2026,54(1):39-52,14.基金项目
2024年鹤壁市重大创新专项 （）
