山西农业科学2026，Vol.54Issue(1)：70-80,11.DOI:10.26942/j.cnki.issn.1002-2481.2026.01.08
小偃麦衍生系抗旱基因功能分子标记检测及效应分析
Detection and Effect Analysis of Functional Molecular Markers of Drought-Resistant Genes in Wheat-Thinopyrum intermedium Introgression Lines
摘要
Abstract
In order to further clarify the favor alleles of drought-resistant gene composition in the wheat-Thinopyrum intermedium introgression lines,in this study,the functional molecular markers of 5 wheat drought resistant genes,including TaNRX-B1,TaDreb-B1,TaPYL1-1B,TaBADH-A1,and TaDRO-5B,were used to detect 175 wheat-Thinopyrum intermedium introgression lines.The drought resistance effect of each allele was analyzed by combining the drought resistance coefficients of 13 drought-resistant indicators and the comprehensive evaluation D value at the germination stage.The results showed that the frequency of the 5 drought-resistant alleles was generally low in the introgression lines,among which TaDreb-B1a had the highest proportion(38.86％)and Hap-5B-I allele of TaDRO-5B gene had the lowest proportion(5.14％).Among the 117 lines with moderate or above drought resistance,CH6310(TaDreb-B1a/TaBADH-A1b/TaPYL1-1BIn-442/Hap-5B-I)and CH1539(TaNRX-B1a/TaDreb-B1a/TaBADH-A1b/Hap-5B-I),carrying 4 drought-resistant alleles,were highly drought-resistant lines.As the five drought-resistant alleles were not detected in the 13 highly drought-resistant lines,there might be new unknown drought-resistant genes.The average drought resistance coefficients of most indicators of introgression lines carrying drought-resistant alleles TaDreb-B1a,TaPYL1-1BIn-442 and Hap-5B-I were higher than those of introgression lines carrying their drought-sensitive alleles.The drought-resistant alleles were significantly positively correlated with the drought resistance coefficients of multiple indicators,and the drought resistance effect was higher.Therefore,the functional molecular markers of drought-resistant genes such as TaDreb-B1,TaPYL1-1B and TaDRO-5B could be effectively used for drought-resistant genotype detection and molecular marker-assisted selection breeding in wheat-Thinopyrum intermedium introgression lines.关键词
小偃麦衍生系/抗旱基因/功能分子标记/等位基因/抗旱系数Key words
wheat-Thinopyrum intermedium introgression line/drought resistant gene/functional molecular marker/al-leles/drought resistance coefficient分类
农业科技引用本文复制引用
常利芳,乔彦玮,郭慧娟,乔麟轶,张树伟,陈芳,李欣,畅志坚,张晓军..小偃麦衍生系抗旱基因功能分子标记检测及效应分析[J].山西农业科学,2026,54(1):70-80,11.基金项目
山西省基础研究计划(202403021211048) （202403021211048）
农业农村部政府购买服务项目(19230606) （19230606）
