上海中医药杂志2026，Vol.60Issue(2)：17-27,11.DOI:10.16305/j.1007-1334.2026.z20250717001
黄连平胃散对溃疡性结肠炎小鼠的治疗效果评价及其作用机制研究
Therapeutic efficacy and underlying mechanisms of Huanglian Pingwei Powder on ulcerative colitis mice
摘要
Abstract
Objective To investigate the therapeutic mechanism of Huanglian Pingwei Powder in treating ulcerative colitis(UC)model.Methods Ultra-Performance Liquid Chromatography-Mass Spectrometry/Mass Spectrometry(UPLC-MS/MS)was used to detect the main components of the drug.The TCMSP and PubChem databases were used to identify the targets of the drug's components.The UC model was established in mice using dextran sulfate sodium(DSS).Metabolomics analysis was conducted to assess the effects of Huanglian Pingwei Powder on metabolites.Hematoxylin and Eosin(HE)staining and Alcian Blue-Periodic Acid-Schiff(AB-PAS)staining were used to observe changes in inflammation and mucus in colon tissue.Immunohistochemistry was performed to detect the expression of Zonula Occludens-1(ZO-1),and Enzyme-Linked Immunosorbent Assay(ELISA)was used to measure serum levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and reactive oxygen species(ROS).Western blot was used to assess the levels of phosphorylated protein 53(p53)and cysteine aspartate-specific protease 3(CASP3)proteins in colon tissue.Molecular docking was used to validate the binding affinity and interaction modes of key compounds with p53 and CASP3.Results Huanglian Pingwei Powder is primarily composed of flavonoids,saccharides and glycosides,phenylpropanoids,alkaloids,and terpenoids.Treatment with this formula regulated serum metabolites such as adenosine monophosphate,guanosine diphosphate-glucose,and indole compounds,significantly reducing serum TNF-α,IL-6,and ROS levels.It also increased expression of ZO-1 and mucin secretion,reduced inflammatory cell infiltration in intestinal tissue,and inhibited p53 and CASP3 protein expression in colon tissue.Both dehydrocorydaline and coptisine exhibited strong binding affinity for p53 and CASP3.Conclusion Huanglian Pingwei Powder alleviates intestinal inflammation and improves gut function by inhibiting the p53 signaling pathway.关键词
溃疡性结肠炎/黄连平胃散/p53信号通路/网络药理学/代谢组学/中药研究Key words
ulcerative colitis/Huanglian Pingwei Powder/p53 signaling pathway/network pharmacology/metabonomics/Chinese materia medica research引用本文复制引用
黄定庸,李志强,刘思文,湛孝东..黄连平胃散对溃疡性结肠炎小鼠的治疗效果评价及其作用机制研究[J].上海中医药杂志,2026,60(2):17-27,11.基金项目
国家自然科学基金项目(82502208) （82502208）
