山西农业大学学报（自然科学版）2026，Vol.46Issue(1)：23-32,10.DOI:10.13842/j.cnki.issn1671-8151.202507054
花椒WRKY基因家族全基因组鉴定及其盐胁迫下表达分析
Genome-wide of the WRKY gene family in Zanthoxylum bungeanum and expression profiling under salt stress
摘要
Abstract
[Objective]WRKY transcription factors are plant-specific regulatory proteins that extensively modulate plant growth,development,secondary metabolism,and response to environmental stressors.While previous studies on the Zanthoxylum bungeanum WRKY family were confined to the transcriptome level,this study performed a comprehensive genome-wide analy-sis to provide a theoretical framework for understanding the functional roles of WRKY genes in this species.[Methods]Using integrated bioinformatics approaches,we systematically identified WRKY gene family members within the Z.bungeanum ge-nome.We characterized their physicochemical properties,phylogenetic relationships,gene structures,conserved motifs,and codon usage bias.Furthermore,we investigated chromosomal distribution,synteny,protein-protein interaction(PPI)net-works,and expression patterns under salt stress.[Results]A total of 289 WRKY genes(ZbWRKY1 to ZbWRKY289)were identified and found to be unevenly distributed across 53 chromosomes.Phylogenetic analysis classified ZbWRKYs into three major subfamilies(I,II,and III),with subfamily II further partitioned into five subgroups.Synteny analysis revealed two pre-dominant orthologous patterns between Z.bungeanum and related species:"one-to-one"(conserved)and"one-to-many"(ex-panded).PPI network modeling highlighted complex functional associations,identifying ZbWRKY109,ZbWRKY217,and ZbWRKY245 as hub proteins with high connectivity.Analysis of Cis-acting elements indicated that ZbWRKY promoters are enriched with motifs responsive to biotic/abiotic stresses,phytohormones,and developmental signals.Additionally,codon us-age bias analysis revealed a strong preference for T and G at the third codon position,primarily driven by natural selection.Based on expression profiling,10 ZbWRKY genes were prioritized as candidate positive or negative regulators of salt stress tol-erance.[Conclusion]The study provided a comprehensive landscape of the evolution and functional diversification of the WRKY gene family in Z.bungeanum and established a foundation for future functional validation and molecular breeding for salt tolerance.关键词
花椒/WRKY基因家族/生物信息学分析/盐胁迫/表达模式Key words
Z.bungeanum/WRKY gene family/Bioinformatics analysis/Salt stress/Expression pattern analysis分类
农业科技引用本文复制引用
王海燕,张馨之,蒋晋豫,赵雅,刘玉林..花椒WRKY基因家族全基因组鉴定及其盐胁迫下表达分析[J].山西农业大学学报（自然科学版）,2026,46(1):23-32,10.基金项目
陕西省林业科技创新计划专项(SXLK2024-0211) （SXLK2024-0211）
