山西农业大学学报(自然科学版)2026,Vol.46Issue(1):33-47,15.DOI:10.13842/j.cnki.issn1671-8151.202506035
欧山葡萄杂交优系'140902'抗抽干性及酿酒特性
Screening of physiological indicators for winter desiccation resistance and study on winemaking charac-teristics of Hybrid wine grape selection'140902'
摘要
Abstract
[Objective]This study aimed to explore the physiological characteristics of winter desiccation resistance and the eno-logical quality of the superior grape line'140902',verifying its feasibility for promotion as a high-quality wine grape in arid and low-temperature areas.[Methods]Using one-year-old shoots of'Beihong'and the desiccation-resistant selection'140902'as materials,physiological and biochemical indicators were studied under natural overwintering conditions to comprehensively evaluate and screen for resistance markers.Based on variation detection,candidate genes related to desiccation resistance were identified via KEGG enrichment analysis.Their roles were further explored through real-time fluorescence quantitative(RT-qPCR)analysis and enzyme activity assays.Meanwhile,berry aroma and quality traits were determined using HS-SPME-GC-MS,followed by sensory analysis to evaluate winemaking characteristics.[Results]Under natural overwintering,the desicca-tion rate of'140902'was significantly lower than that of'Beihong'.The shoot desiccation rate correlated significantly with sev-en indicators,including total pectin and cellulose content.Principal Component Analysis extracted four principal components,representing 92.04%of the original data.Membership function analysis showed that the average comprehensive evaluation D-values for'140902'and'Beihong'were 0.641 and 0.534,respectively.KEGG analysis identified three desiccation-related can-didate genes with significantly different expression patterns between the two genotypes.Furthermore,'140902'berries exhibit-ed significantly higher tannins,total phenols,and total acid content than'Beihong',with an aroma profile dominated by green and fruity notes.[Conclusion]Seven indicators,including total pectin,CAT,and POD activity,can serve as key indicators for assessing desiccation resistance.Compared to'Beihong','140902'exhibited superior desiccation resistance and strong wine typicity,making it a promising high-quality selection for arid and semi-arid regions.关键词
品系/抽干/酿酒葡萄/总果胶含量/香气Key words
New strain/Winter desiccation/Wine grape/Total pectin content/Aroma分类
农业科技引用本文复制引用
董锦,包小琴,姜兴荣,王志磊,徐国前..欧山葡萄杂交优系'140902'抗抽干性及酿酒特性[J].山西农业大学学报(自然科学版),2026,46(1):33-47,15.基金项目
宁夏回族自治区自然科学基金(2023AAC02027) (2023AAC02027)