山西农业大学学报（自然科学版）2026，Vol.46Issue(1)：48-59,12.DOI:10.13842/j.cnki.issn1671-8151.202506025
基于根叶性状研究不同林龄油松对氮添加的响应策略
The response strategies of Pinus tabulaeformis at different stand ages to nitrogen addition based on leaf and root traits
摘要
Abstract
[Objective]While the role of plant functional traits in predicting interspecific ecological strategies is well-established,evidence regarding their ability to predict intraspecific strategies across different growth stages remains limited.This study aims to explore how variations in root and leaf functional traits drive the adjustment of ecological strategies in Pinus tabuliformis for-ests at different growth stages and to reveal the adaptive mechanisms of these strategies to nitrogen(N)addition.[Methods]In this study,natural secondary forests of P.tabuliformis(20 and 40 years old)in the Zhongcun Forestry Station in Zhongtiao Mountains were selected as the research objects.Three N-addition treatments were established:without N addition(CK,0),low-concentration N(LN,5 g N/(m2·a))and high-concentration N(HN,15 g N/(m2·a)).The study measured a variety of leaf traits,including specific leaf area(SLA),leaf dry matter content(LDMC),and carbon(C),N,and phosphorus(P)con-centrations,and their stoichiometric ratios.Root traits including root diameter,specific root length(SRL),root tissue density(RTD),physiological traits such as root respiration rate(RR)and root organic acid content(ROA),and chemical traits like root C,N,and P concentrations,were also measured.Additionally,mycorrhizal fungal traits including mycorrhizal coloniza-tion rate(MC)and hyphal length density(HLD)were investigated.[Results](1)Stand age,N treatment,and their interac-tions had significant effects on almost all leaf and mycorrhizal traits(P<0.05).The 20-year-old P.tabuliformis exhibited high-er leaf C and P concentrations,RTD,MC,and HLD.Below-ground resource acquisition in the 20-year-old forest relied pri-marily on mycorrhizal fungi,with the trees themselves tending toward a conservative strategy.In contrast,the 40-year-old for-est showed higher SLA,SRL,RR,ROA,and root N and P concentrations,revealing a resource-acquisition strategy.Further-more,a significant and positive correlation was found between SLA and SRL(P<0.05)showing synergy between above-ground and below-ground resource acquisition.(2)Compared to the CK,both low and high N treatments significantly in-creased SLA and SRL in both stand ages.In the 20-year-old forest,SLA increased by 9.3%and 39.9%,and SRL increased by 15.2%and 15.5%,respectively.In the 40-year-old forest,these traits increased by 13.3%and 13.0%(SLA),and 7.9%and 8.6%(SRL).Conversely,MC rates significantly decreased across the board(by 35.9%and 28.0%in the 20-year-old forest;24.9%and 17.9%in the 40-year-old forest).N addition also significantly increased leaf N and P concentra-tions in the 20-year-old forest but decreased leaf and root N concentrations in the 40-year-old forest.[Conclusion]Although both 20-and 40-year-old P.tabuliformis forests were in rapid growth stages,the 20-year-old forest relies mainly on mycorrhizal hy-phae for below-ground resource acquisition,employing a conservative strategy.The 40-year-old forest demonstrated a syner-gistic above-and below-ground resource-acquisition strategy.Nitrogen addition prompted P.tabuliformis to enhance functional traits related to leaf and root resource acquisition while simultaneously reducing dependence on mycorrhizal colonization.关键词
生存策略/功能性状/菌根/氮添加/油松/林龄Key words
Survival strategy/Functional traits/Mycorrhiza/Nitrogen addition/P.tabulaeformis/Stand age分类
农业科技引用本文复制引用
孙剑,梁文佳,梁佳芳,杨秀云,刘碧桃..基于根叶性状研究不同林龄油松对氮添加的响应策略[J].山西农业大学学报（自然科学版）,2026,46(1):48-59,12.基金项目
山西省基础研究计划(自由探索类)面上项目(202103021224157) （自由探索类）
山西农业大学横向科技项目(2023QT190) （2023QT190）
