山西农业大学学报（自然科学版）2026，Vol.46Issue(1)：90-98,9.DOI:10.13842/j.cnki.issn1671-8151.202508023
松球壳孢拮抗细菌ZG7的分离与鉴定
Isolation and identification of the antagonistic bacterium ZG7 against Sphaeropsis sapinea
摘要
Abstract
[Objective]Shoot blight caused by Sphaeropsis sapinea is one of the major diseases affecting Pinus sylvestris var.mongolica.This study aimed to isolate and screen superior endophytic antagonistic bacteria from the host to provide novel strategies and methodologies for the biological control of this disease.[Methods]Endophytic bacteria with antagonistic effects against S.sapinea were screened from different tissues of P.sylvestris var.mongolica using the dual culture technique.Poten-tial isolates were identified through morphological,physiological,and biochemical characterization,as well as molecular biolo-gy methods.The antagonistic mechanism against S.sapinea was observed via scanning electron microscopy(SEM).Addition-ally,the antimicrobial spectrum,optimal fermentation conditions,and potential plant growth-promoting effects on the host were determined.[Results]An endophytic bacterial strain,designated ZG7,was isolated from the stem tissue of P.sylvestris var.mongolica.This strain exhibited significant antagonistic activity against S.sapinea,with an inhibition rate of 83.57%.Strain ZG7 was identified as Bacillus amyloliquefaciens.SEM observations revealed that ZG7 induced severe morphological ab-normalities in S.sapinea hyphae,including swelling,distortion,breakage,shrinkage,and increased branching.The strain demonstrated broad-spectrum antagonistic activity against eight tested phytopathogens,with inhibition rates exceeding 53%,the highest being against Colletotrichum gloeosporioides(80.12%).Furthermore,strain ZG7 exhibited nitrogen-fixing capa-bility and produced indole-3-acetic acid(IAA).Optimal fermentation conditions were determined as:temperature 37℃,initial pH 6.0,1%(v/v)inoculum size,and 10%(v/v)medium volume(10 mL per 100 mL flask).[Conclusion]B.amyloliquefa-ciens ZG7 exhibited significant antagonism against S.sapinea,a broad antimicrobial spectrum,and potential growth-promot-ing capabilities.These findings provided a theoretical foundation for the integrated management of shoot blight in P.sylvestris var.mongolica.关键词
解淀粉芽孢杆菌/松球壳孢/樟子松枯梢病/生物防治Key words
B.amyloliquefaciens/S.sapinea/Shoot blight of P.sylvestris var.mongolica/Biocontrol分类
农业科技引用本文复制引用
郭婧,付思娜,贺澳门,周博如..松球壳孢拮抗细菌ZG7的分离与鉴定[J].山西农业大学学报（自然科学版）,2026,46(1):90-98,9.基金项目
国家重点研发计划项目(22YFD1401005) （22YFD1401005）
