陕西师范大学学报（自然科学版）2026，Vol.54Issue(1)：9-17,9.DOI:10.15983/j.cnki.jsnu.2026002
基于双链DNA-银纳米簇/金纳米棒构建荧光适体探针用于微囊藻毒素-LR传感分析
A fluorescent aptamer probe for detection of microcystin-LR based on double-stranded DNA-silver nanoclusters/gold nanorods
摘要
Abstract
A fluorescent aptamer probe for highly sensitive sensing detection of microcystin-LR(MC-LR)was developed based on double-stranded DNA-silver nanoclusters(dsDNA-AgNCs)coupled with gold nanorods(AuNRs).Three DNA nucleotides were designed in this work,including one MC-LR aptamer chain and two C-riched single stranded DNA S1 and S2 complementary to aptamer.Single stranded DNA-AgNCs(ssDNA-AgNCs)with red fluorescence were synthesized by using single stranded DNA S1 and S2 as templates and reduction of silver ions(Ag+)with sodium borohydride(NaBH4).Two ssDNA-AgNCs hybridized with aptamer strands at its two ends to form negatively charged dsDNA-AgNCs with a maximum fluorescence emission at 624 nm.In the presence of positively charged AuNRs,fluorescent resonance energy transfer(FRET)occurred between dsDNA-AgNCs and AuNRs due to the electrostatic interaction,resulting in fluorescent quenching of dsDNA-AgNCs.After addition of the target MC-LR,MC-LR specifically bonded with aptamer of dsDNA-AgNCs,resulting in dissociation of dsDNA-AgNCs to ssDNA-AgNCs and restoring of fluorescence intensity.An"off-on"fluorescent aptamer probe was constructed for MC-LR quantitative detection.The proposed method exhibited a linear response for MC-LR detection in the concentration range of 5 ng/L to 500 μg/L with a detection limit of 1.7 ng/L.The proposed fluorescent aptamer probe possesses the advantages of simple preparation and high selectivity and sensitivity,and can be applied for the MC-LR detection in actual water samples.关键词
微囊藻毒素-LR/双链DNA-银纳米簇/金纳米棒/荧光适体探针Key words
microcystin-LR/double-stranded DNA-silver nanoclusters/gold nanorods/fluorescent aptamer probe分类
化学化工引用本文复制引用
李军,罗焰,阿华英,张艳丽,高连训,王红斌,杨文荣,庞鹏飞..基于双链DNA-银纳米簇/金纳米棒构建荧光适体探针用于微囊藻毒素-LR传感分析[J].陕西师范大学学报（自然科学版）,2026,54(1):9-17,9.基金项目
国家自然科学基金(21565031,21665027) （21565031,21665027）
云南省科技厅应用基础研究基金(202001AT070012) （202001AT070012）
云南民族大学研究生科研创新基金(2023SKY028) （2023SKY028）
