中国草食动物科学2026，Vol.46Issue(1)：72-78,7.DOI:10.3969/j.issn.2095-3887.2025.00.050
牦牛PSTPIP2和SLC6A18基因多态性与免疫性状的关联分析
Association Analysis of PSTPIP2 and SLC6A18 Gene Polymorphisms with Immune Traits in Yak
摘要
Abstract
PSTPIP2 and SLC6A18 genes play an important role in innate autoimmunity.In order to investigate the asso-ciation between the polymorphisms at the g.43165308C＞T locus of PSTPIP2 gene and the g.8294767T＞C locus of SLC6A18 gene with immune traits in yaks,in this study,the PSTPIP2 gene g.43165308C＞T locus and the SLC6A18 g.8294767 T＞C locus were amplified by PCR in 189 Niangya yaks,and using the PARMS SNP genoty-ping.The correlation between different gene loci and immune traits such as immunoglobulin,inflammatory mar-kers,and cytokines in yaks was analyzed using IBM SPSS Statistics 26.0 software.The results showed that there were CC,CT,and TT genotypes at the g.43165308C＞T locus of PSTPIP2 gene,and the dominant allele and genotype were C and CC,respectively,with frequencies of 0.720 and 0.534,respectively.The PSTPIP2 gene g.43165308C＞T locus was moderately polymorphic and in Hardy-Weinberg equilibrium(P＞0.05).There were three genotypes of CC,TC and TT at the g.8294767T＞C locus of SLC6A18 gene,and the dominant allele and genotype were T and TT,respectively,with frequencies of 0.796 and 0.660,respectively.The g.8294767T＞C locus of SLC6A18 gene was a moderate polymorphism and was in a Hardy-Weinberg imbalance state(P＜0.05).The results of association analysis showed that the g.43165308C＞T locus of PSTPIP2 gene was significantly correlated with IgA,IgM,CRP,IL2,IL4,IL6 and TNF-α in yak(P＜0.05).The g.8294767T＞C locus of SLC6A18 gene was significantly cor-related with CRP,IL4 and IFN-γ(P＜0.05).In summary,the polymorphisms of PSTPIP2 and SLC6A18 genes were significantly related to yak immune traits,and the two genes may be potential candidate genes affecting yak immune traits.关键词
牦牛/PSTPIP2基因/SLC6A18基因/免疫性状Key words
Yak/PSTPIP2 genes/SLC6A18 genes/immune traits分类
农业科技引用本文复制引用
杨焱,张娟香,洛桑顿珠,喇永富,马晓明,卓玛次仁,尼玛加措,平措占堆,梁春年..牦牛PSTPIP2和SLC6A18基因多态性与免疫性状的关联分析[J].中国草食动物科学,2026,46(1):72-78,7.基金项目
西藏自治区区域科技协同创新专项(QYATZX-RKZ2022-03) （QYATZX-RKZ2022-03）
西藏自治区重点研发计划(XZ202402ZY0012) （XZ202402ZY0012）
现代肉牛牦牛产业技术体系(CARS-37) （CARS-37）
甘肃省科技计划项目(23CXNA0011) （23CXNA0011）
