中国动物检疫2026，Vol.43Issue(1)：24-33,10.DOI:10.3969/j.issn.1005-944X.2026.01.005
山东省和广东省部分地区不同动物源大肠杆菌耐药及毒力基因检测
Detection of Antibiotic Resistance and Virulence Genes of Different Animal-Derived Escherichia coli in Selected Regions in Shandong and Guangdong Provinces
摘要
Abstract
In order to investigate the distribution of antibiotic resistance and virulence genes of different animal-derived Escherichia coli(E.coli)in southern and northern China,a total of 201 samples were collected from different animals suspected of E.coli infection on several farms in Shandong Province and Guangdong Province,for isolation and identification of the strain,followed by antibiotic susceptibility testing and detection of antibiotic resistance and virulence genes.The results showed that a total of 193 strains of E.coli were isolated and identified,with an isolation rate of 96.02％,specifically,35.23％for fly-derived strains,29.02％for layer hen-derived strains,24.35％for goose-derived strains,4.66％for each rabbit-and sheep-derived strains,and 2.07％for broiler chicken-derived strains.89.64％of strains were resistant to multiple drugs,especially to tetracycline,nalidixic acid and trimethoprim,with the resistance rate above 70.00％,moderately to amikacin(46.11％)and flumequine(47.15％),but sensitive to tigecycline,with a resistance rate of 1.04％.28 antibiotic resistance genes were detected in the identified E.coli strains,the carriage rate was high in floR(44.56％),tetA(19.69％)and aadA1(16.58％),but 9.33％in mcr1 that was clinically significant;16 virulence genes were detected,with a high detection rate in phoA(33.16％)and ropS(31.09％).The resistance rate and the detection rate of virulence genes were different among different animals,the former was 100％,and the latter was 75.00％and 44.11％for broiler chicken-and fly-derived strains,respectively,while the former was lower(70.21％and 66.67％),and the latter was higher(89.36％and 88.89％)for geese-and sheep-derived strains.In conclusion,different animal-derived E.coli isolated from the two provinces generally presented strong antibiotic resistance,with diverse resistance and virulence genes and high detection rate.The distribution of antibiotic resistance and virulence genes in multiple animal-derived E.coli was revealed in the study,and served as a data support for future development of local antimicrobial stewardship programs and resistance control strategies.关键词
大肠杆菌/不同动物源/耐药基因/毒力基因Key words
E.coli/different animal-derived/drug resistance gene/virulence gene分类
农业科技引用本文复制引用
柳妍玲,赵永达,段笑笑,原培勋,郭莉莉,付来宇,王群义,张一驰,李彦,王欢,宋亮,徐靖雯,陈佳文..山东省和广东省部分地区不同动物源大肠杆菌耐药及毒力基因检测[J].中国动物检疫,2026,43(1):24-33,10.基金项目
山东省重点研发计划项目(2024CXGC010910) （2024CXGC010910）
山东省泰山产业领军人才项目(TS20220701) （TS20220701）
中央引导地方资金项目(YDZX2023123) （YDZX2023123）
青岛市产业培育计划科技惠民专项(24-1-8-xdny-4-nsh,25-1-5-xdny-30-nsh) （24-1-8-xdny-4-nsh,25-1-5-xdny-30-nsh）
