中国动物检疫2026，Vol.43Issue(1)：34-42,9.DOI:10.3969/j.issn.1005-944X.2026.01.006
致急性肝胰腺坏死病欧文氏弧菌的鉴定与分析
Identification and Analysis of Vibrio owensii Causing Acute Hepatopancreatic Necrosis Disease
摘要
Abstract
Shrimp farming industry has been severely threatened by acute hepatopancreatic necrosis disease(AHPND)that is one of shrimp diseases.In order to thoroughly investigate the pathogenic characteristics of the disease,preliminary pathogen screening and isolation identification were conducted using fluorescent PCR assay based on a batch of shrimp larvae suspected of AHPND,followed by gene sequencing,phylogenetic analysis and pathogenicity testing targeting the isolated strain.The results showed that the shrimp larvae were co-infected with AHPND-causing bacteria and Enterocytozoon hepatopenaei(EHP);yellow colonies were produced in TCBS medium by the isolated strain,and identified as non-Vibrio parahaemolyticus,all its tested genes including PirA,PirB,AP3 and AP4 were positive;and it was found that,by phylogenetic analysis of 16S rRNA,the isolated strain clustered with Vibrio owensii.Gene sequencing showed that complete pirA and pirB toxin genes were contained in the PVA1 plasmid of the isolated strain.Pathogenicity testing revealed that,when SPF Litopenaeus vannamei were immersed in a 106 CFU/mL solution of the isolated strain,typical AHPND symptoms appeared in their hepatopancreas,with a cumulative mortality rate approaching 60％within 48 hours post-infection,while that of VPAHPND control group was 100％.In conclusion,AHPND could also be caused by non-Vibrio owensii where corresponding virulence genes were carried,though its virulence differed from that of VPAHPND.Future researches on shrimp pathogen diversity and pathogenicity,as well as the efforts for disease control were supported by the data obtained in this study.关键词
急性肝胰腺坏死病/肝肠胞虫/欧文氏弧菌/毒素基因/致病性Key words
AHPND/EHP/Vibrio owensii/toxin gene/pathogenicity分类
农业科技引用本文复制引用
李家侨,斯泽恩,刘荭,刘骁,杨劲,谢艳辉,杨金金,唐媛媛,张娜..致急性肝胰腺坏死病欧文氏弧菌的鉴定与分析[J].中国动物检疫,2026,43(1):34-42,9.基金项目
海关总署科研项目(2024HK029,2023HK037) （2024HK029,2023HK037）
湛江海关科研项目(2024JHK010) （2024JHK010）
