中国临床药理学杂志2025，Vol.41Issue(23)：3343-3349,7.DOI:10.13699/j.cnki.1001-6821.2025.23.007
金丝桃苷抑制NF-κB和NLRP3促进糖尿病足溃疡大鼠创面愈合的研究
Research of hyperoside inhibits NF-κB and NLRP3 to promote wound healing in diabetic foot ulcer rats
摘要
Abstract
Objective To investigate the promoting effect and mechanism of hyperoside(Hyp)on wound healing in diabetic foot ulcer(DFU)rats by inhibiting the nuclear factor-kappa B(NF-κB)pathway and the Nod-like receptor family Pyrin domain-containing 3(NLRP3)inflammasome.Methods A total of 50 SD rats were randomly divided into blank group,model group,metformin group,low-dose experimental group and high-dose experimental group,with 10 rats in each group.Fasting blood glucose(FBG),wound healing rate and transcutaneous oxygen partial pressure(TcpO2)in the tissues surrounding the wounds were measured using a blood glucose meter.Pathological changes in the wound tissues were observed using hematoxylin-eosin(HE)staining.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum levels of interleukin(IL)-8,IL-1 β,tumor necrosis factor-alpha(TNF-α)and C-reactive protein(CRP).Western blotting was employed to assess the relative expression levels of matrix metalloproteinase 9(MMP-9),tissue inhibitor of metalloproteinase 1(TIMP-1),NF-κB p65,phosphorylated(p)-NF-κB p65 and NLRP3 proteins.Results The FBG levels in blank,model,metformin,low-dose experimental and high-dose experimental groups were(5.31±0.91),(22.52±3.18),(11.27±2.35),(13.11±2.67)and(8.76±1.15)mmol·L-1,respectively;the wound healing rates were(76.91±12.30)％,(9.53±1.01)％,(45.26±6.15)％,(39.94±5.47)％and(57.10±9.26)％,respectively;the TcpO2 values were(41.09±8.51),(24.35±4.97),(33.27±5.68),(30.84±3.26),and(37.92±4.14)mmHg,respectively;the serum levels of IL-8 were(15.34±0.39),(41.92±3.12),(26.68±2.28),(30.55±3.27)and(17.95±2.70)ng·L-1,respectively;IL-1 β levels were(31.05±4.62),(64.39±7.14),(46.62±5.07),(50.21±3.92)and(39.54±4.25)ng·L-1,respectively;TNF-α levels were(26.95±4.15),(69.74±7.43),(39.85±4.29),(45.08±4.36)and(35.52±2.95)ng·L-1,respectively;and CRP levels were(0.69±0.08),(5.11±0.60),(3.23±0.58),(3.68±0.71)and(1.95±0.30)ng·L-1,respectively;the protein relative expression levels of MMP-9 were 0.23±0.03,0.99±0.11,0.62±0.05,0.74±0.06 and 0.39±0.04,respectively;the MMP-9/TIMP-1 ratios were(22.56±1.23)％,(97.07±5.67)％,(60.18±5.01)％,(70.49±5.98)％and(38.39±4.15)％,respectively;the p-NF-κB p65/NF-κB p65 protein relative expression levels were 0.21±0.02,1.23±0.10,0.51±0.04,0.57±0.06 and 0.33±0.04,respectively;and the NLRP3 protein relative expression levels were 0.25±0.01,0.93±0.07,0.72±0.08,0.80±0.06 and 0.35±0.04,respectively.Statistically significant differences were observed when comparing the model group with the blank group and the low-and high-dose experimental groups with the model group(all P＜0.05).Conclusion Hyp promotes wound healing in DFU rats by inhibiting NF-κB and NLRP3 pathways,reducing inflammation and reducing MMP-9 expression.关键词
金丝桃苷/糖尿病足溃疡/核因子κB/Nod样受体家族Pyrin结构域3/基质金属蛋白酶9/创面愈合Key words
hyperoside/diabetic foot ulcer/nuclear factor-κB/Nod-like receptor family pyrin domain 3/matrix metalloproteinase 9/wound healing分类
医药卫生引用本文复制引用
朱秋霄,刘子渤,树林一,郝志华,郝慧瑶..金丝桃苷抑制NF-κB和NLRP3促进糖尿病足溃疡大鼠创面愈合的研究[J].中国临床药理学杂志,2025,41(23):3343-3349,7.基金项目
河北省2021年度医学科学研究课题基金资助项目(20210516) （20210516）
