中国临床药理学杂志2025，Vol.41Issue(23)：3350-3357,8.DOI:10.13699/j.cnki.1001-6821.2025.23.008
基于PI3K/AKT通路探究四君子汤改善T2DM大鼠胰腺部位胰岛素抵抗的机制研究
Exploring the mechanism of Sijunzi decoction ameliorates pancreatic insulin resist-ance in T2DM rats:A focus on the PI3K/AKT pathway
摘要
Abstract
Objective To investigate the effect and underlying mechanism of Sijunzi Decoction in ameliorating insulin resistance(IR)within the pancreatic islets of rats with type 2 diabetes mellitus(T2DM).Methods A total of 40 Sprague-Dawley(SD)rats were randomly divided into control group(n=10,fed with normal diet)and high-sugar and high-fat group[n=30,fed with high-sugar and high-fat diet coupled with intraperitoneal injection of streptozotocin(STZ)to create a type 2 diabetes mellitus(T2DM)model].The smoothly modeled SD rats were further randomly assigned into model group,positive control group(0.003 g·mL-1)and experimental group(0.848 g·mL-1).The normal group and model group were given distilled water by gavage,while the other two groups were administered the corresponding drugs by gavage for 4 consecutive weeks.Fasting blood glucose(FBG)was measured using a blood glucose meter.Serum levels of fasting insulin(FINS),total cholesterol(TC),total triglyceride(TG),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)in rats of each group were detected with commercial assay kits.The pancreatic index(PI)was calculated based on the detection of pancreatic tissue.Reverse transcription-polymerase chain reaction(RT-PCR)was employed to determine the mRNA expression levels of related proteins,and Western blotting was performed to ascertain the formulation standards of correlated total proteins.Results The FBG,FINS,TC,TG,HDL-C,LDL-C and PI in the model group and experimental group were(13.60±0.61)mmol·L-1 vs.(9.73±0.43)mmol·L-1,(15.24±0.76)mIU·L-1vs.(12.39±1.13)mIU·L-1,(5.42±0.41)mmol·L-1 vs.(3.76±0.26)mmol·L-1,(1.49±0.26)mmol·L-1 vs.(0.65±0.05)mmol·L-1,(0.82±0.06)mmol·L-1 vs.(1.12±0.08)mmol·L-1,(4.82±0.43)mmol·L-1 vs.(3.02±0.42)mmol·L-1,and(0.27±0.09)％vs.(0.34±0.02)％,respectively.The mRNA expression levels of INSR,IRS-1,PI3K,AKT,GLUT4,GSK-3β,and FOXO1 were 0.44±0.16 vs.0.61±0.16,0.48±0.13 vs.0.72±0.16,0.62±0.30 vs.0.84±0.12,0.74±0.20 vs.0.92±0.02,0.48±0.22 vs.0.78±0.24,1.90±0.16 vs.1.56±0.25,and 1.83±0.16 vs.1.60±0.21,respectively.The total protein expression levels of INSR,IRS-1,PI3K,AKT,GLUT4,GSK-3 β,and FOXO1 were 0.59±0.29 vs.0.82±0.18,0.65±0.28 vs.0.72±0.16,0.49±0.14 vs.0.74±0.15,0.50±0.17 vs.0.68±0.12,0.65±0.28 vs.0.86±0.14,2.73±0.19 vs.2.50±0.22,and 1.61±0.49 vs.1.21±0.35,respectively.Compared with the model group,all the above indicators in the experimental group showed statistically significant differences(P＜0.001,P＜0.01,P＜0.05).Conclusion Sijunzi Decoction can improve pancreatic insulin resistance(IR)in rats with type 2 diabetes mellitus(T2DM)by regulating the expression of 7 signaling factors in the upstream,middle and downstream of the PI3 K/AKT signaling pathway.关键词
四君子汤/2型糖尿病/PI3K/AKT通路/胰腺/胰岛素抵抗Key words
Sijunzi decoction/T2DM/PI3K/AKT pathway/pancreas/insulin resistance分类
医药卫生引用本文复制引用
杜雪珊,薛春龙,杨少博,张玺雅,任杰,段秀俊..基于PI3K/AKT通路探究四君子汤改善T2DM大鼠胰腺部位胰岛素抵抗的机制研究[J].中国临床药理学杂志,2025,41(23):3350-3357,8.基金项目
山西省自然科学研究面上基金资助项目(202203021211214) （202203021211214）
山西中医药大学科技创新能力培训计划"太行本草"专项基础和应用研究基金资助项目(2022PY-TH-51) （2022PY-TH-51）
