中国农业科学2026，Vol.59Issue(2)：265-277,13.DOI:10.3864/j.issn.0578-1752.2026.02.004
外源硝普钠对碱胁迫下水稻幼苗糖代谢的影响
The Effect of Exogenous Nitroprusside on Sugar Metabolism in Rice Seedlings Under Alkaline Stress
摘要
Abstract
[Objective]Sodium nitroprusside(SNP),as an exogenous nitric oxide(NO)donor,plays a critical regulatory role in plant stress responses.This study aimed to investigate the effects of exogenous SNP on sugar metabolism in rice seedlings under alkaline stress,for providing a theoretical basis for elucidating the mechanism of SNP enhancing rice alkali tolerance.[Method]Seedlings of rice cultivars Zhonghua 11 and Ningjing 52 were used as test materials.Four treatments were applied:control(CK),CK+SNP(50 µmol·L-1),alkaline stress(AS,a mixed alkaline solution containing NaHCO3 and Na2CO3 at a 1:1 ratio,pH 9.55,20 mmol·L-1),and AS+SNP(20 mmol·L-1 AS+50 µmol·L-1 SNP).The effects of these treatments on sugar metabolism in the seedlings of the two rice cultivars were analyzed.[Result]Compared with AS treatment,AS+SNP treatment significantly increased rice seedling height,root length,fresh weight,and dry weight of rice seedlings.AS+SNP treatment upregulated the expression of chlorophyll synthase-related genes(OsChlH,OsCAO1,and OsCAO2),thereby increasing chlorophyll content and promoting photosynthesis.Concurrently,it upregulated the expression of the sucrose invertase gene OsNIN1,leading to decreased fructose and sucrose content but increased glucose content,which facilitated glycolysis and the tricarboxylic acid cycle,promoting the accumulation of malate and citrate to maintain energy balance.Additionally,SNP induced the expression of sucrose phosphate synthase OsSPS1,gibberellin synthase-related genes(OsGA3ox2,and OsGA20ox1),and proline synthesis gene(OsP5CS),increasing the levels of gibberellin(GA),abscisic acid(ABA),proline(Pro),and soluble sugar.This regulation helped maintain osmotic and hormonal balance and promoted sucrose transport from source to sink tissues.[Conclusion]Exogenous SNP application enhanced rice seedling tolerance to alkaline stress by upregulating chlorophyll biosynthesis genes,promoting chlorophyll accumulation,and sustaining photosynthetic efficiency,thereby facilitating glucose synthesis.Moreover,SNP activated glycolysis and the tricarboxylic acid cycle,directing sugar metabolites toward malate and citrate production,thus promoting organic acid accumulation.Additionally,exogenous SNP modulated osmotic regulation and GA/ABA hormonal balance,facilitating sucrose transport and sugar metabolic pathway activation,and ensuring efficient energy metabolism.These mechanisms collectively improved rice seedling resilience under alkaline stress.关键词
NO/碱胁迫/糖代谢/基因表达/生理响应/水稻Key words
NO/alkaline stress/sugar metabolism/gene expression/physiological response/rice引用本文复制引用
廖婷璐,石亚飞,肖东浩,舍杨梦斐,郭富城,杨九菊,唐海江,罗成科..外源硝普钠对碱胁迫下水稻幼苗糖代谢的影响[J].中国农业科学,2026,59(2):265-277,13.基金项目
宁夏自然科学基金(2025AAC030188)、宁夏科技创新团队项目(2022BSB03109)、宁夏重点研发计划(2024BBF0100102) （2025AAC030188）
