中国畜牧兽医2026，Vol.53Issue(2)：521-531,11.DOI:10.16431/j.cnki.1671-7236.2026.02.001
家畜卵母细胞玻璃化冷冻保护剂应用进展与优化策略
Application Advance and Optimization Strategies in Cryoprotectant Agents for Vitrification of Livestock Oocytes
摘要
Abstract
Oocyte cryopreservation is a vital technology for preserving germplasm resources of genetically superior livestock and endangered species,and also serving as a critical tool for breed improvement and commercial embryo production.As essential agents in vitrification,cryoprotectant agents protect cells by lowering the freezing point,increasing viscosity,preventing ice crystallization,and mitigating osmotic damage.However,the cytotoxicity of cryoprotectant agents remains one of the critical challenges to be addressed in vitrification technology.The development of novel cryoprotectant agents with low toxicity and high efficacy has become a key research direction for improving the efficiency of livestock oocyte vitrification.This review systematically analyzes the cytotoxic mechanisms of conventional cryoprotectant agents in oocytes preservation,with particular emphasis on recent advances in novel cryoprotective agents,including small-molecule permeable cryoprotectants,lipid modulators,antioxidants,and cytoskeleton stabilizers.It will provide both theoretical basis and technical references for optimizing the vitrification systems of livestock oocytes.关键词
卵母细胞/玻璃化冷冻/冷冻损伤/冷冻保护剂Key words
oocytes/vitrification/cryoinjury/cryoprotectant agents分类
农业科技引用本文复制引用
李伉,陈思颍,孙雅雯,冷璇,王栋,庞云渭..家畜卵母细胞玻璃化冷冻保护剂应用进展与优化策略[J].中国畜牧兽医,2026,53(2):521-531,11.基金项目
国家重点研发计划(2021YFD1200301) （2021YFD1200301）
