中国畜牧兽医2026，Vol.53Issue(2)：532-542,11.DOI:10.16431/j.cnki.1671-7236.2026.02.002
窦前卵泡体外培养在反刍动物中的研究进展
Research Progress on in vitro Culture of Preantral Follicles in Ruminants
摘要
Abstract
Preantral follicles represent a critical stage in the transition from follicular quiescence to active growth and are abundant in the ovaries of ruminants,possessing high developmental potential and significant application value.With the advancement of reproductive biotechnology,in vitro culture of preantral follicles has become an important focus in ruminant reproductive biology as an assisted reproductive approach.This technique not only contributes to improve livestock reproductive efficiency but also provides new strategies for the preservation of genetic resources and population restoration of endangered species.However,the in vitro development of ruminant preantral follicles is still constrained by multiple factors,such as the structural complexity of follicles,instability of culture systems,and low oocyte developmental competence,which limit its further application.Ruminant preantral follicles can achieve a certain degree of growth and development under in vitro conditions,and some studies have obtained early embryos such as morulae,but the overall maturation rate and developmental potential remain limited.This review systematically summarizes the isolation methods and in vitro culture systems of ruminant preantral follicles,including mechanical and enzymatic isolation,in situ culture,two-dimensional culture,and three-dimensional culture,and compares the differences among these methods and systems in terms of follicular integrity,applicability,and developmental performance.Future studies should focus on the standardization of isolation methods,optimization of culture systems,and elucidation of the mechanisms underlying long-term follicle survival and in vitro maturation,with the goal of establishing more efficient and stable culture systems and promoting the application of this technology in livestock production and the conservation of endangered species.关键词
反刍动物/窦前卵泡/体外培养/分离技术Key words
ruminants/preantral follicles/in vitro culture/isolation techniques分类
农业科技引用本文复制引用
郭芷函,李阳,王绍雄,韩志强,李肖,崔冰冰,吕洁,张光磊,徐超..窦前卵泡体外培养在反刍动物中的研究进展[J].中国畜牧兽医,2026,53(2):532-542,11.基金项目
吉林省梅花鹿产业技术体系(JLARS-2025-080203) （JLARS-2025-080203）
吉林梅花鹿种质资源库(20250402004ZP) （20250402004ZP）
