种子2025，Vol.44Issue(12)：40-47,54,9.DOI:10.16590/j.cnki.1001-4705.2025.12.040
薏苡根的代谢组与转录组联合分析
Integrated Analysis of Transcriptome and Metabolome in Coix lacryma-jobi Roots
摘要
Abstract
The Coix lacryma-jobi seed is an important coarse grain crop and traditional Chinese medicine,rich in lipid-like active substances.However,the key genes and regulatory pathways that affect lipid synthesis in Coix lacryma-jobi seed roots remained unclear.In order to the regulatory mechanism of lipid synthesis metabolism in Coix lacryma-jobi roots,metabolomics and transcriptomics techniques were utilized to detect lipid metabolites and expressed genes at 3 growth stages(tillering stage,tasseling stage,and maturity stage)of Coix lacryma-jobi seed roots,and to analyze the differential metabolites and differentially expressed genes of roots at different growth stages.The results showed that the metabolomics analysis of Coix lacryma-jobi seed roots detected a total of 103 differential metabolites,among which fatty acids and derivatives were the main types,containing 21 kinds,accounting for 20.39％of the total metabolites.Transcrip to mic analysis screened out a total of 21 528 differentially expressed genes.Among them,5 058 genes were up-regulated,16 470 genes were down-regulated,and 344 genes were differentially expressed in all three reproductive peri-ods.GO annotations indicated that differentially expressed genes were mainly involved in biological processes,while KEGG annotations suggested that they were mainly involved in pathways such as lipid metabolism,carbohydrate metabolism,and energy metabolism.Joint analysis revealed that 2 key enzyme genes were sig-nificantly correlated with lipid synthesis and accumulation,and might be the key genes regulating lipid metab-olism in Coix lacryma-jobi seed root.Meanwhile,during the tillering stage of Coix lacryma-jobi seed root,metabolites such as Caprylicacid,Pyruvicacid,and(R)-3-hydroxybutyric acid(R)-3-hydroxybutyric acid accumulated the most D-Mannose and Cis-Aconitic Acid accumulate the most during the heading stage,while Gamma-Aminobutyric Acid(GABA)accumulated the highest amount during the maturation stage.关键词
薏苡/脂质代谢/代谢组学/转录组学Key words
Coix lacryma-jobi/lipid metabolism/metabolomics/transcriptomics分类
农业科技引用本文复制引用
李秀诗,石丹丹,付瑜华,尚昆,蒙秋伊,杨玲玲..薏苡根的代谢组与转录组联合分析[J].种子,2025,44(12):40-47,54,9.基金项目
国家自然科学基金(No.32360480) （No.32360480）
贵州省科技计划项目(黔科合基础-ZK[2023]一般183) （黔科合基础-ZK[2023]一般183）
贵州省农业科学院项目(黔农科院青年科技基金[2020]06号、黔农科种质资源[2023]08号和黔农科GJHX[2025]01号) （黔农科院青年科技基金[2020]06号、黔农科种质资源[2023]08号和黔农科GJHX[2025]01号）
贵州省特色杂粮生物育种全省重点实验室(黔科合平台ZSYS[2025]026) （黔科合平台ZSYS[2025]026）
