种子2025，Vol.44Issue(12)：48-54,7.DOI:10.16590/j.cnki.1001-4705.2025.12.048
5个高粱品系染色体制片技术优化及核型分析
Optimization of Chromosome Preparation and Karyotype Analysis in 5 Strains of Sorghum bicolor
摘要
Abstract
To explore the karyotype characteristics of different strains of Sorghum bicolor,the enzy-matic hydrolysis titration method was used to conduct karyotype analysis on 5 strains,namely Jiniang No.1,Desheng Sweep Broom Sorghum,Jinnuo No.3,Jingdu Xiaobairen and Jinbai No.29.The results showed that mitotic metaphase chromosomes could be obtained by fixation w ith 90％glacial acetic acid and Carnoy solution respectively using the root tips of Sorghum bicolor as the material.After pretreatment with a mixture of ice and water and fixation with Carnoy solution,the chromo-some preparation effect was better,and the obtained chromosomes had clear morphology,moderate shrinkage and uniform dispersion.In this study,all 5 sorghum plants were diploid(2n=2x=20),all had karyotype 1A,and each had a pair of chromosome with satellite structures.The karyotype formu-la of Jiniang No.1 was 2n=2x=18m(2SAT)+2sm,that of Desheng Sweep Broom Sorghum was 2n=2x=2M+18m(2SAT),and the karyotype formulas of the other strains were all 2n=2x=20m(2SAT).The results of the karyotype asymmetry coefficient showed that their evolutionary degrees from high to low were as follows:Jinbai No.29,Jiniang No.1,Desheng Sweep Broom Sorghum,Jing-du Xiaobairen and Jinnuo No.3.关键词
高粱/核型分析/染色体制片技术/细胞学Key words
Sorghum bicolor/karyotype analysis/chromosome preparation techniques/cytology分类
农业科技引用本文复制引用
曹瑶,王琳琳,张继苗,郑钺..5个高粱品系染色体制片技术优化及核型分析[J].种子,2025,44(12):48-54,7.基金项目
国家自然科学基金项目"基因组组成不同的芸苔属异源三倍体减数分裂行为差异和育性形成机理研究"(32401882) （32401882）
山西省基础研究计划项目"异源三倍体油菜ACC花粉育性降低的细胞学机理研究"(202403021222242) （202403021222242）
