种子2025，Vol.44Issue(12)：66-75,10.DOI:10.16590/j.cnki.1001-4705.2025.12.066
不同硒浓度下水稻的转录组和代谢组分析
Transcriptomic and Metabolomic Analysis of Rice under Different Selenium Concentrations
摘要
Abstract
After being absorbed by plants,selenium can affect their physiological activities,gene expression and metabolite content,etc.,and thus influence plant growth.At present,the molecular mechanism by which rice(Oryza sativa L.)responds to selenium stress was still not perfect.The effects of different seleni-um concentrations on the growth and metabolism of rice were systematically studied through multi-omics analysis methods such as phenotypic analysis,transcriptome sequencing,metabolomics analysis and KEGG pathway enrichment.The results showed that when the selenium concentration was 8 μmol/L,it had a sig-nificant promoting effect on the growth of rice.However,when the selenium concentration exceeded 12μmol/L,the growth of rice plants was inhibited.Transcriptome analysis indicated that selenium could regu-late the growth and development and stress response of rice by influencing photosynthesis,metabolic activi-ties,and signaling pathways.Metabolomics analysis indicated that selenium treatment mainly had significant effects on organic acids,phenolic acids,lipids,terpenoids,alkaloids,amino acids and their derivatives in rice leaves.At low concentrations,the synergistic enrichment of the pentose phosphate pathway,the TCA cycle and glycolysis could enhance energy metabolism and reducing power supply,enabling rice to achieve adaptive growth to the selenium environment.At high concentrations,there was a significant enrichment of cysteine/methionine metabolism(selenium methylation detoxification),phenylpropane/flavonoid synthesis(phenolic antioxidant barrier),alanine-aspartate-glutamic acid metabolism(providing glutathione precursor),glyine-serine metabolism(transporting methyl donors),and starch sucrose metabolism(osmotic regulation and car-bon source storage),which indicated that plants could convert selenium into volatile dimethyl selenoether through sulfur metabolism reprogramming,and at the same time shiftted the carbon flow from energy pro-duction to the synthesis of secondary metabolites,constructing a'detoxification-antioxidation-osmotic protec-tion'trinity defense network.These results were of great significance for revealing the molecular mechanism of rice's response to selenium stress.关键词
水稻/硒/转录组/代谢组Key words
Oryza sativa L./selenim/transcriptome/metabolome分类
农业科技引用本文复制引用
刘立超,门龙楠,魏中华,孙中华,宗天鹏,沙汉景,谢树鹏..不同硒浓度下水稻的转录组和代谢组分析[J].种子,2025,44(12):66-75,10.基金项目
东北北部苏打盐碱地资源高效和耐盐碱水稻新品种设计与培育(2023ZD040660104) （2023ZD040660104）
优质耐碱水稻新品种创制(ZY04JD05-005) （ZY04JD05-005）
中晚熟优质特种稻新品种选育推广与产业化开发(CX23ZD02-05) （CX23ZD02-05）
抗甲咪唑烟酸水稻种质资源利用(2020FJZX031) （2020FJZX031）
水稻种质资源创新与新品种选育(SHFY2022-06) （SHFY2022-06）
东北稻区抗病粳稻新品种培育与推广(2023ZD040660104) （2023ZD040660104）
黑龙江省现代农业产业技术协同创新推广体系 （）
利用CRISPR/Cas9创制优异香型水稻种质资源(CX25JC44) （CX25JC44）
