种子2025，Vol.44Issue(12)：76-83,8.DOI:10.16590/j.cnki.1001-4705.2025.12.076
臭氧水浸种对玉米种子活力的影响及转录组分析
Effect of Ozone Water Soaking on Maize Seed Vigor and Its Transcriptome Analysis
摘要
Abstract
To explore the effect of ozone water immersion on the vitality of maize(Zea mays L.)seeds and its molecular mechanism,using the maize inbred line B73 as the material,the seeds were soaked in deionized water and ozone water for 12 hours respectively,and then cultivated in a constant temper-ature climate chamber.The activities of antioxidant enzymes were determined at 0 days,1.5 days and 4 days of seed germination.Meanwhile,transcriptome sequencing was performed to screen for differ-entially expressed genes,and GO and KEGG enrichment analyses were carried out.The results showed that,compared with the control,ozone water treatment significantly increased the germina-tion rate,germination potential,germination index,root length and bud length of maize seeds.The activities of CAT,POD and SOD in ozone water treatment were significantly higher than those in the control group at 0 days,1.5 days and 4.0 days.Transcriptome analysis revealed that there were 4 972 differentially expressed genes(3 327 up-regulated and 1 645 down-regulated)between the two treat-ment groups,mainly enriched in GO items such as REDOX enzyme activity,membrane protein com-plexes,and secondary metabolite synthesis,as well as KEGG pathways such as indole alkaloid bio-synthesis.Studies had shown that ozone water soaking of seeds could affect the biosynthesis of indole alkaloids by regulating the activity of antioxidant enzymes,thereby enhancing the vitality of maize seeds.关键词
臭氧水/玉米种子/种子活力/转录组分析Key words
ozone water/maize seeds/seed vigor/transcriptome analysis分类
农业科技引用本文复制引用
熊婷,王欣妹,陈佳,马浩然,李光祖,张姿丽,蒋锋,刘鹏飞..臭氧水浸种对玉米种子活力的影响及转录组分析[J].种子,2025,44(12):76-83,8.基金项目
2023年广东省普通高校自然科学类重点领域专项(2023ZDZX4017) （2023ZDZX4017）
广东省2024年省级乡村振兴战略专项资金种业振兴项目(2024-NJS-00-005) （2024-NJS-00-005）
广东省阳江市2023年省科技创新专项资金(SDZX2023024) （SDZX2023024）
广东省现代农业产业技术体系创新团队建设项目(2024CXTD07) （2024CXTD07）
