浙江大学学报（农业与生命科学版）2026，Vol.52Issue(1)：27-40,14.DOI:10.3785/j.issn.1008-9209.2024.12.242
工业大麻CsGASAs基因家族鉴定及表达分析
Identification and expression analysis of CsGASAs gene family in industrial hemp(Cannabis sativa)
摘要
Abstract
Gibberellin-regulated protein(GASA)plays a significant role in plant growth,development and stress resistance.In this study,we used the industrial hemp(Cannabis sativa)cultivar'Longma 5'as the material to identify and characterize the CsGASAs gene family,aiming to elucidate its regulatory mechanisms in industrial hemp growth,development,and stress physiology.Using Arabidopsis thaliana GASA family sequences as a reference,the transcriptome data of CsGASA genes in industrial hemp were screened via the National Center for Biotechnology Information(NCBI)database and bioinformatics tools such as TBtools.A total of 11 CsGASAs family members were identified.Bioinformatics analysis revealed that all CsGASAs were hydrophilic and were predicted to be located in the Golgi apparatus,cytoplasm,or nucleus.Gene structure analysis indicated that the CsGASAs contained two untranslated regions and two to four coding sequences.Promoter cis-acting element analysis identified various regulatory elements,including light-responsive elements and hormone-responsive elements related to abscisic acid(ABA),auxin and gibberellin A3(GA3).The expression patterns of CsGASAs under exogenous phytohormone treatments[e.g.,GA3,ABA,salicylic acid(SA)]and drought stress were analyzed using quantitative reverse transcription polymerase chain reaction(qRT-PCR).The results showed that this gene family was regulated by drought stress and multiple phytohormones,with the highest expression levels on the 8th day of drought stress or after SA treatment.Further analysis revealed that the expression levels of CsGASA1 and CsGASA8 were significantly upregulated on the 8th day of drought stress,indicating that they may play a role in the stress response.Based on the presence of ABA-responsive elements,it was speculated that CsGASAs may participate in the drought stress response via the ABA signal pathway.Additionally,we successfully cloned the CsGASA6 gene,which had a coding sequence length of 345 bp,encoding 115 amino acids.Sequencing confirmed 99.64%homology with the reference sequence in the NCBI database.An overexpression vector for the CsGASA6 gene was constructed,and transgenic Arabidopsis thaliana plants were successfully obtained via Agrobacterium-mediated transformation.Integrated bioinformatics and qRT-PCR results confirm that the CsGASAs gene family in industrial hemp is highly conserved and may be involved in drought stress response and hormone signal transduction,potentially enhancing plant stress resistance.关键词
工业大麻/CsGASAs基因家族/激素处理/非生物胁迫/表达分析Key words
industrial hemp(Cannabis sativa)/CsGASAs gene family/hormone treatment/abiotic stress/expression analysis分类
农业科技引用本文复制引用
杨森,王雪松,邵庆一,曹佳昂,王欣悦,赵楠,王嫱,李珊珊,刘丽杰..工业大麻CsGASAs基因家族鉴定及表达分析[J].浙江大学学报（农业与生命科学版）,2026,52(1):27-40,14.基金项目
黑龙江省省属本科高校基本科研业务费项目(145309512) （145309512）
齐齐哈尔大学研究生创新科研项目(QUZLTS_CX2024016,QUZLTS_CX2025066). （QUZLTS_CX2024016,QUZLTS_CX2025066）
