浙江大学学报（农业与生命科学版）2026，Vol.52Issue(1)：63-72,10.DOI:10.3785/j.issn.1008-9209.2025.01.061
沉默AiPRMT1基因降低小地老虎对辛硫磷和氯虫苯甲酰胺的耐受性
Silencing of AiPRMT1 gene reduces tolerance to phoxim and chloran-traniliprole in Agrotis ipsilon
摘要
Abstract
Protein arginine methyltransferase(PRMT)plays crucial roles in insect growth,development,repro-duction,and circadian rhythm regulation.However,the role of PRMT in insecticide tolerance remains poorly understood.In this study,a PRMT gene(designated AiPRMT1)was identified from the black cutworm(Agrotis ipsilon),an important agricultural pest.The sequence characteristics of AiPRMT1 were analyzed via bioinformatic tools.The transcriptional pattern of AiPRMT1 was examined through real time fluorogenic quantitative PCR.AiPRMT1 was silenced via RNA interference technology,and the tolerance of A.ipsilon larvae to three insecticides(phoxim,lambda-cyhalothrin,and chlorantraniliprole)was assessed.The results showed that AiPRMT1 consisted of eight exons and seven introns,and the length of the open reading frame was 1 071 bp.The protein encoded by AiPRMT1 displayed typical characteristics of PRMT.Phylogenetic analysis revealed that AiPRMT1 was most closely related to HaPRMT1 from Helicoverpa armigera and SlPRMT1 from Spodoptera litura.Expression profiling analysis indicated that AiPRMT1 had the highest transcription level in the larval fat body,and the transcription level was higher in the second-to third-instar larval stages than in other developmental stages.The transcriptional levels of AiPRMT1 were obviously upregulated after treatment with phoxim,lambda-cyhalothrin,or chlorantraniliprole.Silencing of AiPRMT1 markedly enhanced larval sensitivity to phoxim and chlorantraniliprole,with mortality rates increasing by 7%and 28%(P＜0.05),respectively,compared with the control group.In contrast,tolerance to lambda-cyhalothrin was not significantly altered.These findings provide a theoretical foundation for further exploration of the molecular mechanisms underlying insecticide tolerance regulated by AiPRMT1.关键词
小地老虎/蛋白质精氨酸甲基转移酶/RNA干扰/杀虫剂耐受性/生物测定Key words
Agrotis ipsilon/protein arginine methyltransferase/RNA interference/insecticide tolerance/bioassay分类
农业科技引用本文复制引用
李响,刘苏,张今,沈望熙,史肖肖,余佳敏,刘东阳,邓全,彭江南,李茂业..沉默AiPRMT1基因降低小地老虎对辛硫磷和氯虫苯甲酰胺的耐受性[J].浙江大学学报（农业与生命科学版）,2026,52(1):63-72,10.基金项目
安徽省教育厅省级质量工程项目(2023xqhz019) （2023xqhz019）
中国烟草总公司四川省公司科技项目(SCYC202410,SCYC202519,SCYC202112) （SCYC202410,SCYC202519,SCYC202112）
烟草行业青年科技托举人才项目(YCQTSC202401) （YCQTSC202401）
国家级大学生创新创业训练计划项目(202410364057) （202410364057）
安徽农业大学校级大学生创新创业训练计划项目(X202410364529). （X202410364529）
