浙江大学学报（农业与生命科学版）2026，Vol.52Issue(1)：73-84,12.DOI:10.3785/j.issn.1008-9209.2025.01.151
通过斑马鱼模型探究多酚类物质抗辣木叶蛋白致敏作用
Exploring the anti-allergic effects of polyphenols against the proteins from Moringa oleifera leaves using zebrafish model
摘要
Abstract
To explore the allergenic effects of Moringa oleifera leaf protein(MLP)and the anti-allergic effects of polyphenols such as lotus leaf polyphenol extract(LPE)and Polygonatum polyphenol extract(PPE),MLP was extracted to construct a zebrafish food allergy model.Zebrafish were simultaneously administered LPE and PPE.The allergenic effects of MLP and the anti-allergic effects of LPE and PPE were investigated by behavioral analysis,intestinal section staining,and real time fluorescent quantitative polymerase chain reaction(qRT-PCR).The results indicated that MLP reduced the locomotor activity of zebrafish,while LPE and PPE could restore this indicator,demonstrating a protective effect.MLP caused significant damage to the intestinal barrier,whereas LPE and PPE enhanced immune responses,promoted intestinal recovery and alleviated allergic symptoms.qRT-PCR result showed that LPE and PPE regulated the expression of inflammatory factors such as interleukin-6(IL-6),interleukin-1β(IL-1β),interleukin-4/13B(IL-4/13B),and interleukin-10(IL-10),thereby inhibiting the inflammatory response induced by MLP.This study confirms the allergenicity of MLP and the anti-allergic potential of LPE and PPE,providing a scientific basis for the prevention and treatment of food allergies.关键词
辣木叶蛋白/荷叶多酚/黄精多酚/斑马鱼模型/抗过敏Key words
Moringa oleifera leaf protein/lotus leaf polyphenols/Polygonatum polyphenols/zebrafish model/anti-allergic分类
轻工纺织引用本文复制引用
郑跃妍,糜波,周溯,黄澄怡,蒋益虹..通过斑马鱼模型探究多酚类物质抗辣木叶蛋白致敏作用[J].浙江大学学报（农业与生命科学版）,2026,52(1):73-84,12.基金项目
国家木薯产业技术体系项目(CARS-11-ZJLBY). （CARS-11-ZJLBY）
