植物保护2026，Vol.52Issue(1)：17-26,78,11.DOI:10.16688/j.zwbh.2025025
大白菜水杨酸通路基因BraNPR1、BraTGA3参与调控芜菁花叶病毒侵染
BraNPR1 and BraTGA3 from the salicylic acid signaling pathway in Chinese cabbage mediate resistance against turnip mosaic virus
摘要
Abstract
Salicylic acid(SA)functioning as a acrucial phytohormone and signaling molecule,which regulates plant immune responses by modulating the expression of defense-related genes such as NPR1,TGA transcription factors.To elucidate the roles of salicylic acid pathway genes in the interaction between Chinese cabbage and viruses,the turnip mosaic virus(TuMV)-susceptible cultivar'Liangqing'and Nicotiana benthamiana were used as plant materials.Through sequence homology and phylogenetic analysis,BraNPR1 and eight BraTGAs in TGA family members were identified in the genome of Chinese cabbage.RT-qPCR analysis revealed that these genes were transcriptionally responsive to TuMV infection.Subcellular localization,yeast two-hybrid,and bimolecular fluorescence complementation assays confirmed that BraNPR1 interacts with BraTGA3 in the nucleus.Additionally,co-infiltration assays in N.benthamiana revealed that overexpression of BraNPR1 and BraTGA3 upregulated the pathogenesis-related protein 1 gene(PR1)and inhibited viral accumulation.In conclusion,these findings demonstrate that BraNPR1 and BraTGA3 function cooperatively in the salicylic acid signaling pathway to mediate antiviral defense against TuMV in Chinese cabbage.关键词
大白菜/芜菁花叶病毒/NPR1/TGA3/相互作用机制Key words
Chinese cabbage/turnip mosaic virus/NPR1/TGA3/interaction mechanism分类
农业科技引用本文复制引用
高维芳,李雪,赵程通,姜露,贾明轩,周雪平,李星,李方方..大白菜水杨酸通路基因BraNPR1、BraTGA3参与调控芜菁花叶病毒侵染[J].植物保护,2026,52(1):17-26,78,11.基金项目
国家自然科学基金(32172385) （32172385）
