植物保护2026，Vol.52Issue(1)：27-38,66,13.DOI:10.16688/j.zwbh.2025018
喜树丛枝植原体基因组组装及功能分析
Genome assembly and functional analysis of Camptotheca acuminata witches'-broom phytoplasma
摘要
Abstract
To elucidate the genome structure of the Camptotheca acuminata witches'-broom phytoplasma,high-throughput sequencing combined with the Phytoassembly pipeline was employed to assemble a draft genome filtered from host genomic data.The assembled genome is 608 891 bp in length and contains 642 coding sequences,32 tRNA genes,and two rRNA operons.Annotation analysis revealed that this phytoplasma lacks several genes related to amino acid and fatty acid biosynthesis,the tricarboxylic acid cycle,oxidative phosphorylation,and the pentose phosphate pathway.However,genes for glycolysis and pyruvate oxidation are intact,suggesting that the phytoplasma mainly depends on these two pathways for energy production.Additionally,the genome is enriched in genes encoding ATP-binding cassette(ABC)transporters,which are predicted to mediate the transport of spermidine/putrescine,dipeptides/oligopeptides,cobalt,manganese,zinc,and amino acids.Notably,the presence of genes encoding malate transporter and malate dehydrogenase indicates that this phytoplasma may utilize host-derived malate as a carbon source for ATP synthesis.Two secreted proteins homologous to the aster yellows phytoplasma effectors SAP11AY-WB and SAP05AY-WB were also identified,suggesting their possible involvement in the induction witches'-broom symptoms.Phylogenetic analysis showed that this phytoplasma is most closely related to'Candidatus Phytoplasma luffae'within the 16Sr Ⅷ group.This study,for the first time,reports the genome sequence of the Camptotheca acuminata witches'-broom phytoplasma,providing a valuable reference for understanding phytoplasma pathogenic mechanisms and advancing genomic research on obligate,difficult-to-culture microorganisms.关键词
喜树丛枝植原体/基因组草图/代谢途径/分泌蛋白Key words
Camptotheca acuminata witches'-broom phytoplasma/genome draft/metabolic pathways/secreted proteins分类
农业科技引用本文复制引用
黄维依,乔开,李学美,周宇杰,苏帆,林木森,蔡红..喜树丛枝植原体基因组组装及功能分析[J].植物保护,2026,52(1):27-38,66,13.基金项目
国家自然科学基金(31960535) （31960535）
