植物保护2026，Vol.52Issue(1)：39-46,8.DOI:10.16688/j.zwbh.2025056
甜菜夜蛾短神经肽F基因调控取食、能量代谢及饥饿耐受性的功能分析
Functional analysis of short neuropeptide F gene regulating feeding,energy metabolism and starvation tolerance in Spodoptera exigua
摘要
Abstract
To clarify regulatory effects of short neuropeptide F(sNPF)gene in Spodoptera exigua on feeding behavior,energy metabolism and starvation tolerance,we cloned and functionally analyzed the sNPF gene.The results showed that the open reading frame(ORF)of the sNPF gene inS.exigua was 548 bp,encoding 182 amino acids.The protein sequence exhibited 96.7％-100％amino acid sequence homology with sNPF proteins from other species of the Noctuidae family within Lepidoptera,demonstrating high conservation.Tissue expression analysis revealed that sNPF was most highly expressed in the fourth-instar larvae,particularly in the head.RNA interference(RNAi)technology(via injection of double-stranded sNPF interfering RNA,dssNPF)was employed,and the results showed that compared with the control group(injected with dsGFP),the feeding amount of fourth-instar larvae was significantly reduced by 13.15％,17.59％and 20.13％at 24,48 and 72 h after interference,respectively.Meanwhile,the cumulative body weight during the same periods decreased significantly by 10.14％,23.08％,and 34.59％,respectively.Further starvation stress experiments revealed that interference with the sNPF gene led to a significant increase in the mortality of fourth-instar larvae,reaching 54％and 100％at 48 and 72 h,respectively.In addition,the glycogen content in the larvae decreased significantly by 37.53％,while the levels of trehalose(increased by 66.42％),trehalose synthase(increased by 24.26％),insulin(increased by 53.59％)all increased significantly.These results suggest that the sNPF gene directly influences the growth,development and starvation tolerance of S.exigua by regulating feeding behavior and energy substance accumulation and metabolism(e.g.,accelerated glycogen consumption and enhanced trehalose synthesis).This gene plays a key role in the survival and adaptation of the insect.The findings provide an important theoretical basis for developing novel and green control technologies targeting the sNPF gene for S.exigua management.关键词
甜菜夜蛾/短神经肽F/取食/糖代谢Key words
Spodoptera exigua/short neuropeptide F/feeding/sugar metabolism分类
农业科技引用本文复制引用
滕海媛,顾浩天,王冬生,张颂函,张天澍,袁永达..甜菜夜蛾短神经肽F基因调控取食、能量代谢及饥饿耐受性的功能分析[J].植物保护,2026,52(1):39-46,8.基金项目
上海市现代农业产业技术体系建设[沪农科产字(2024)第1号] （2024）
