植物保护2026，Vol.52Issue(1)：90-102,13.DOI:10.16688/j.zwbh.2025050
Cry1Ac抗性棉铃虫对转Bt基因玉米的敏感性变化及机制研究
Sensitivity changes and mechanisms of Cry1Ac-resistant cotton bollworm to transgenic Bt maize
摘要
Abstract
Transgenic insect-resistant crops can effectively control target pests,however,with prolonged cultivation,the risk of pests developing resistance increases,thus,assessing pest resistance risk in advance and developing rational resistance management strategies are crucial for the sustainable application of transgenic crops.In this study,the sensitivity changes of Cry1Ac-resistant Helicoverpa armigera strains(LF10,LF30,LF60,LF120)feeding on three transgenic Bt maize cultivars('DBN9936''DBN3601T''Ruifeng 125')were determined using the diet incorporation bioassay.The transcriptional levels of major Bt receptor genes were compared between larvae fed on conventional maize and those fed on Bt maize using quantitative real-time PCR(RT-qPCR).The results indicated that the Cry1Ac-resistant strains exhibited reduced sensitivity to all three Bt maize cultivars compared with the susceptible strain,and the sensitivity declined with increasing Cry1Ac resistance level in the resistant strains.Feeding on Bt maize altered the expression of Bt receptor genes in H.armigera,with varying effects among maize cultivars and between different insect strains.Overall,the transcription of ABCC2 was down-regulated after fed on transgenic Bt maize.When feeding on the same Bt maize,resistant H.armigera showed significantly lower transcription levels of ABCC2 and CAD than the susceptible strain.These results suggest that ABCC2 may play a major role in the stress response of H.armigera under Bt protein exposure.The Cry1Ac-resistant H.armigera showed obvious cross-resistance to the three Bt maize cultivars(which express Cry1Ab proteins),and the significant down-regulation of ABCC2 and CAD in resistant strains may be the main molecular basis for the cross-resistance.关键词
棉铃虫/转基因抗虫玉米/Bt/交互抗性/机制Key words
Helicoverpa armigera/transgenic insect-resistant maize/Bacillus thuringiensis/cross resistance/mechanism分类
农业科技引用本文复制引用
于欢,汤金荣,房风云,王文汇,张彩虹,李国平,梁革梅..Cry1Ac抗性棉铃虫对转Bt基因玉米的敏感性变化及机制研究[J].植物保护,2026,52(1):90-102,13.基金项目
农业生物育种国家科技重大专项(2022ZD04021) （2022ZD04021）
河南省农业科学院基础性科研工作项目(2023SWYZ01) （2023SWYZ01）
