植物学报2026，Vol.61Issue(1)：37-52,16.DOI:10.11983/CBB25005
牡丹TCP基因家族鉴定及TCP15a功能分析
Identification of TCP Gene Family and Functional Analysis of TCP15a of Tree Peony
摘要
Abstract
INTRODUCTION:Flower color is one of the important traits to evaluate the ornamental value of tree peony(Paeonia suffruticosa).Anthocyanin plays a major role in the color of tree peony petals.The biosynthesis of anthocyanin is mainly controlled by two types of genes which are directly involved in pigment metabolism and transcription factors which play a regulatory role.TCP transcription factors are involved in the regulation of secondary metabolism,flavonoid and chlorophyll synthesis in plants.Therefore,it is of great value for further analysis of anthocyanin biosynthesis and regulation mecha-nism to explore the gene function of TCP family in tree peony and clarify the regulation mechanism of TCP in tree peony petal coloration. RATIONALE:In order to study the role of TCP transcription factor family in the process of tree peony petal coloration and provide candidate genes for tree peony flower color improvement and molecular breeding,the TCP family of tree peony was identified and analyzed by bioinformatics,and the function of genes TCP13 and TCP15a that may be involved in flower color regulation was analyzed. RESULTS:Twenty-six TCP gene family members were identified from three tree peony genomes and divided into two subtribes and three subclasses,which were irregularly distributed on 5 chromosomes.The physicochemical properties and gene structure of different members were different,but all members contained TCP conserved domains.The transient expression of TCP13 and TCP15a,which may be involved in petal coloration,showed that TCP13 had no obvious effect on the purple phenotype induced by MYB57,while TCP15a could significantly inhibit it.Fluorescence quantitative PCR analysis showed that TCP15a could significantly inhibit the expression of anthocyanin synthase genes CHSa,DFR and ANS in the tissue of disseminated petals.The interaction between TCP15a and MBW protein complex members WD40-1 and WD40-2 was identified by protein interaction identification. CONCLUSION:In this study,twenty-six TCP gene family members were screened and identified by mining the tree peo-ny genome database,and the characteristics of TCP encoded amino acids,evolutionary relationship and gene structure were analyzed by bioinformatics.It was found that TCP15a may inhibit the biosynthesis of anthocyanins by competing with MYB57 and interacting with WD40,regulating the expression of enzyme genes CHSa,DFR and ANS,etc.The re-sults provided a theoretical basis for exploring the molecular mechanism of tree peony petal coloration.关键词
牡丹/花色/TCP转录因子家族/功能分析Key words
tree peony/flower color/TCP transcription factor family/functional analysis引用本文复制引用
韩新蕊,袁欣,高杰,王亮生,王晓晖,贾文庆,符真珠,张和臣..牡丹TCP基因家族鉴定及TCP15a功能分析[J].植物学报,2026,61(1):37-52,16.基金项目
国家自然科学基金(No.32030095)、河南省联合基金(No.242301420128)、河南省农业科学院创新团队(No.2024TD36)、河南省科技攻关项目(No.242102110269)和中央引导计划(No.Z20241471128) （No.32030095）
