中国现代中药2026，Vol.28Issue(1)：74-85,中插14,13.DOI:10.13313/j.issn.1673-4890.20250905006
基于UHPLC-Q-Exactive Orbitrap MS、指纹图谱及多成分含量测定的铁线透骨草质量评价与提取工艺优化
Quality Evaluation and Extraction Process Optimization of Clematis intricata Based on UHPLC-Q-Exactive Orbitrap MS,Fingerprinting,and Multi-component Content Determination
摘要
Abstract
Objective:To analyze the chemical constituents of Clematis intricata,establish its fingerprint profile and a quantitative determination method for four components(chlorogenic acid,caffeic acid,rutin,and paeonol),and evaluate the quality of C.intricata from different geographical origins by combining chemometric analysis.Methods:The chemical constituents of C.intricata were analyzed using ultra-high-performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap MS).A chemical fingerprint was established based on high-performance liquid chromatography(HPLC),and similarity evaluation was performed.Cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)were applied to evaluate the quality of C.intricata.Using extraction solvent,solid-to-liquid ratio,and ultrasonication time as influencing factors,the contents of chlorogenic acid,caffeic acid,rutin,and paeonol were determined,and the extraction process was optimized using an L9(34)orthogonal experimental design.Results:A total of 50 compounds,including flavonoids and phenylpropanoids,were identified in C.intricata,among which five components were identified by comparison with reference standards.Fifteen common peaks were identified in 16 batches of samples using the HPLC fingerprint.Chlorogenic acid and rutin were characteristic components of samples from the Zhangjiakou-Baoding production area,while Peak 5,Peak 2,Peak 1,and Peak 15 were associated with samples from the Chengde region.The optimal extraction conditions for chlorogenic acid,caffeic acid,rutin,and paeonol were 50%ethanol(100 mL)with ultrasonication for 15 min.The four components exhibited good linearity within their respective concentration ranges.The average recovery ranged from 92%to 104%,with RSD values less than 4%.The content determination method complied with the requirements of the Pharmacopoeia of the People's Republic of China(2025 Edition).Conclusion:The established HPLC fingerprint and multi-component quantitative determination method for C.intricata are rational and reliable.When combined with chemometric analysis,they can effectively evaluate the quality of C.intricata from Hebei Province and provide a reference for its quality control.关键词
铁线透骨草/化学成分分析/高效液相色谱法指纹图谱/正交试验设计/含量测定/质量评价Key words
Clematis intricata Bunge/chemical composition analysis/HPLC fingerprint/orthogonal experimental design/content determination/quality assessment分类
医药卫生引用本文复制引用
徐梦婷,赵春雨,周舒,黄文奕,郭彦杰,杜守颖,白洁..基于UHPLC-Q-Exactive Orbitrap MS、指纹图谱及多成分含量测定的铁线透骨草质量评价与提取工艺优化[J].中国现代中药,2026,28(1):74-85,中插14,13.基金项目
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023272) （zyyzdxk-2023272）
