遵义医科大学学报2026，Vol.49Issue(1)：7-12,20,7.
异氟烷对中央杏仁核CRH神经元的激活效应及其空间分布特征研究
A study on the activating effects of isoflurane on CRH neurons in the central a-mygdala and their spatial distribution characteristics
摘要
Abstract
Objective The aim of this study was to investigate the isoflurane-activated cells in the central amyg-dala(CeA)and their relationship with corticotropin-releasing hormone(CRH)neurons.Methods Eight healthy male CRH-tdTomato-Cre mice were randomly assigned to two groups:the isoflurane group(1.4％isoflurane an-esthesia for 2 hours,n=4)and the air control group(n=4).After the experiment,cardiac perfusion was per-formed to collect the brains,and frozen sections of the CeA region were prepared.Immunofluorescence staining was then carried out,and fluorescence microscopy was used for observation and image acquisition.The number of isoflurane-activated cells and the distribution and co-localization of CRH neurons in different layers of the CeA were statistically analyzed.Results Compared to the air control group,the isoflurane group showed a significant increase in neuronal activity in the CeA,with a notable increase in c-Fos-positive cells(P＜0.05).Some of the isoflurane-activated cells were CRH neurons,but the co-localization rate was low.The activated cells were pri-marily located in the posterior regions of the CeA,while CRH neurons were predominantly located in the anterior regions of the CeA.Conclusion Isoflurane significantly activates neurons in the CeA,but only a small proportion of these activated cells are CRH neurons,with most of the activated cells not being CRH neurons.关键词
全身麻醉/异氟烷/中央杏仁核Key words
general anesthesia/isoflurane/central amygdala分类
医药卫生引用本文复制引用
何应丹,白亮,刘继,王智敏,陈西祖,罗天元,余守洋..异氟烷对中央杏仁核CRH神经元的激活效应及其空间分布特征研究[J].遵义医科大学学报,2026,49(1):7-12,20,7.基金项目
国家自然科学基金资助项目(NO:82160682) （NO:82160682）
贵州省科技计划项目[NO:黔科合基础-ZK(2022)一般618] （2022）
贵州省卫生健康委科学技术基金资助项目(NO:gzwkj2021-272). （NO:gzwkj2021-272）
