遵义医科大学学报2026，Vol.49Issue(1)：29-39,11.
CTRP12调控平滑肌细胞表型转化在腹主动脉瘤中的功能研究
Functional study of CTRP12 in regulating vascular smooth muscle cell pheno-typic switching during abdominal aortic aneurysm pathogenesis
摘要
Abstract
Objective This study aimed to investigate the role of C1q/TNF-related protein-12(CTRP12)in Ab-dominal Aortic Aneurysm(AAA)and its underlying molecular mechanisms,with a focus on its effects on inflam-matory factors,matrix metalloproteinases(MMPs),and VSMC phenotypic switching.Methods Serum samples were collected from AAA patients and healthy volunteers,and CTRP12 levels were measured by ELISA.An AAA model was established in ApoE-/-mice via Angiotensin Ⅱ(Ang Ⅱ)infusion using osmotic minipumps.Adeno-associated virus was administered via tail vein injection to modulate CTRP12 expression.Pathological changes in the abdominal aorta were assessed by hematoxylin-eosin(HE)and Masson staining.The expression levels of CTRP12,inflammatory factors(IL-6,IL-1β,TNF-α),MMPs(MMP2,MMP9),and VSMC pheno-typic markers in the abdominal aorta of AAA mice were analyzed using qPCR and Western blot.Results CTRP12 expression was significantly elevated in the serum of AAA patients.In ApoE-/-mice,CTRP12 expression was markedly increased in both serum and abdominal aortic tissues,accompanied by elevated concentrations of IL-6,IL-1β,and TNF-α,and upregulation of MMP2 and MMP9.Knockdown of CTRP12 expression led to reduced levels of these inflammatory factors,downregulated MMP2 and MMP9 expression,and inhibited VSMC phenotyp-ic switching.Conclusion CTRP12 may accelerate AAA progression by regulating inflammatory responses,MMPs expression,and VSMC phenotypic switching.关键词
腹主动脉瘤/C1q/TNF-相关蛋白12/炎症因子/基质金属蛋白酶/血管平滑肌细胞Key words
abdominal aortic aneurysm/C1q/TNF-related protein 12/inflammatory factors/matrix metallo-proteinases/vascular smooth muscle cells分类
医药卫生引用本文复制引用
宋扬,刘达兴,曹依然,韦姗,徐琨尧,王波,罗亮亮,杨焱淼,张建,张登沈..CTRP12调控平滑肌细胞表型转化在腹主动脉瘤中的功能研究[J].遵义医科大学学报,2026,49(1):29-39,11.基金项目
贵州省科技计划项目[NO:黔科合基础-ZK(2022)一般652] （2022）
贵州省卫健委基金资助项目(NO:gzwkj2023-157) （NO:gzwkj2023-157）
遵义医科大学附属医院博士科研启动基金资助项目(NO:院字2021-4). （NO:院字2021-4）
