北京林业大学学报2026，Vol.48Issue(2)：155-164,10.DOI:10.12171/j.1000-1522.20250344
寒旱逆境下不同类型转基因小黑杨生长差异与评价
Growth differences and evaluation of different types of transgenic Populus simonii×P. nigra under cold and drought stress
摘要
Abstract
[Objective]This study aimed to evaluate the growth adaptability and physiological response mechanisms of transgenic Populus simonii×P.nigra clones(with TaLEA,betA,and PsnWRKY70 genes)under cold and drought stress,to screen genotypes combining fast growth with high stress resistance,thereby providing candidate materials for poplar breeding in cold and arid regions of China.[Method]An environmental release trial was established at Cuohai Forest Farm in Heilongjiang Province(cold-temperate,semi-arid climate).Field observations were conducted on 5-year-old transgenic lines and wild-type(WT)controls.Growth traits including tree height,diameter at breast height(DBH),stem volume,and survival rate were measured.During the typical drought period,physiological indicators including malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,hydrogen peroxide(H2O2)content,and stomatal density were analyzed.Analysis of variance(ANOVA),multiple comparisons,and the membership function method were used for comprehensive evaluation and selection.[Result]There were significant differences(P<0.05)in tree height,DBH,volume,and survival rate among the tested clones.With a comprehensive evaluation value≥0.70 as the screening threshold,4 excellent clones were selected,including 3 PsnWRKY70 suppression expression clones(b23,b17,b15)and 1 TaLEA overexpression clone(XL-7).Among them,clone b23 performed the best,with a tree height of 6.14 m and a survival rate of 72.5%.Under drought conditions,clone b23 exhibited low MDA content,high SOD activity,low H2O2 accumulation,and low leaf stomatal density,showing strong resistance to oxidative stress and water retention capacity.Clone XL-7 also showed significantly better growth and stress resistance than the control.[Conclusion]Suppressing PsnWRKY70 expression significantly enhance the field growth and survival ability of Populus simonii×Populus nigra in cold and arid conditions,primarily by boosting antioxidant capacity and cell membrane stability.The selected transgenic clones(b23,b17,b15,and XL-7),combining fast growth with high stress resistance,provide promising new germplasm for stress-resistant poplar breeding in cold,arid regions.关键词
转基因小黑杨/PsnWRKY70/TaLEA/betA/寒旱逆境/生长差异/抗逆育种Key words
transgenic Populus simonii×P. nigra/PsnWRKY70/TaLEA/betA/cold and drought stress/growth difference/stress-resistant breeding分类
农业科技引用本文复制引用
王木廷,胡一杰,董新宇,刘杰,张杰,姜静,李慧玉..寒旱逆境下不同类型转基因小黑杨生长差异与评价[J].北京林业大学学报,2026,48(2):155-164,10.基金项目
农业生物育种国家科技重大专项(2023ZD04056). （2023ZD04056）
