重庆医学2026，Vol.55Issue(1)：1-8,13,9.DOI:10.3969/j.issn.1671-8348.2026.01.001
基于mSOD1转基因小鼠模型对肌萎缩侧索硬化症特征蛋白的鉴定及验证
Identification and validation of characteristic proteins of amyotrophic lateral sclerosis based on mSOD1 transgenic mouse model
摘要
Abstract
Objective To identify and validate the characteristic proteins in the development progres-sion of amyotrophic lateral sclerosis(ALS)based on the mutant superoxide dismutase 1(mSOD1)transgenic mouse model.Methods The mSOD1 transgenic mouse model was constructed,and the proteomics was used to detect the protein expression profile differences in the brain tissues and muscle tissues between the ALS group and wild-type mice(WT group).The enrichment analysis,WGCNA analysis and PPI network construc-tion were conducted.RT-qPCR,Western blot and immunohistochemistry were used to conduct the verifica-tion.Results The mSOD1 transgenic mouse ALS model was successfully established.The differential analysis showed that there was significantly difference in protein expression in the brain and muscle tissues between the MLS group and Wt group.WGCNA showed that the Turquoise protein module was associated with ALS occurrence,and the PPI analysis results showed that chaperonin containing TCP1-subunit 2(CCT2),recombi-nant human phospho-stress-inducible protein 1(STIP1)and heat shock protein 70kDa protein 1A(HSPA1A)might be the characteristic proteins in ALS development process.The RT-qPCR results showed that CCT2,STIP1 and HSPA1A in the ALS group were higher than those in the WT group,and the difference was statis-tically significant(P<0.05).Conclusion CCT2,STIP1 and HSPA1A may be the characteristic proteins in the developing process of ALS caused by mSOD1 mutation.关键词
肌萎缩侧索硬化症/突变型超氧化物歧化酶1/伴侣蛋白含TCP1亚基2/应激诱导磷酸化蛋白1/热休克蛋白家族A成员1AKey words
amyotrophic lateral sclerosis/mutant superoxide dismutase 1/chaperonin containing TCP1-subunit 2/recombinant human phospho-stress-inducible protein 1/heat shock protein 70kDa protein 1A分类
医药卫生引用本文复制引用
吴虹辰,周子豪,张洪兰,胡俊..基于mSOD1转基因小鼠模型对肌萎缩侧索硬化症特征蛋白的鉴定及验证[J].重庆医学,2026,55(1):1-8,13,9.基金项目
重庆市自然科学基金项目(2021-cstc2021jcyj-msxmX0685) （2021-cstc2021jcyj-msxmX0685）
重庆市自然科学基金创新发展联合基金重点项目(CSTB2023NSCQ-LZX0139). （CSTB2023NSCQ-LZX0139）
