重庆医学2026，Vol.55Issue(1)：57-61,5.DOI:10.3969/j.issn.1671-8348.2026.01.010
尿液热带念珠菌qPCR快速检测方法的建立与临床应用价值
Establishment and clinical application of quantitative PCR rapid detection method for urine Candida tropicalis
摘要
Abstract
Objective To establish a quantitative PCR(qPCR)rapid detection method for urine Candi-da tropicalis,and to evaluate its clinical application value.Methods The specific primers were designed on the intra-transcriptional spacer 1(ITS1)between the ribosomal RNA-coding genes(rDNA)of Candida tropicalis.The detection method for Candida tropicalis was constructed by the qPCR analysis system;this method was e-valuated by the limit of detection(LoD),limit of quantitation(LloQ)and analytical specificity.Its clinical ap-plication was evaluated by the urine sample detection.Results The LoD and LloQ of the method for pure bac-terial liquid were 102 CFU/mL.The LoD and LloQ of the method for urine samples were 103 CFU/mL.All u-rine specimens containing Candida tropicalis had amplification and had no amplification for the urine samples with negative culture or containing other pathogenic bacteria.Conclusion This study has successfully con-structed the qPCR rapid detection method for urine Candida tropicalis with low LloQ and high specificity.关键词
念珠菌尿/热带念珠菌/实时荧光定量PCR/内转录间隔序列Key words
candiduria/Candida tropicalis/real-time fluorescence quantitative PCR/internal transcrip-tional spacer sequence分类
医药卫生引用本文复制引用
沈菁,李孝辉,孙静芳,陈燕,徐银海,郭毅..尿液热带念珠菌qPCR快速检测方法的建立与临床应用价值[J].重庆医学,2026,55(1):57-61,5.基金项目
国家自然科学基金青年科学基金项目(82402722) （82402722）
徐州市科技项目2023年度医药卫生面上项目(KC23265). （KC23265）
