东北林业大学学报2026，Vol.54Issue(2)：27-35,9.
榆属种质资源遗传多样性分析及核心种质筛选
Analysis of Genetic Diversity and Core Germplasm Screening of Ulmus Germplasm Resources
摘要
Abstract
Using 15 pairs of self-designed and screened universal EST-SSR primers for Ulmus,we analyzed the genetic diversity and population structure of 184 Ulmus germplasm resources,including 6 species and 2 varieties.Additionally,we con-structed the core germplasm of Ulmus pumila with Nei's genetic distance among individuals as the primary control condi-tion.The results showed that the observed heterozygosity,expected heterozygosity,and unbiased expected heterozygosity of Ulmus germplasm resources were 0.439,0.590,and 0.591,respectively,with a Shannon's information index of 1.278,indicating rich genetic diversity.The 184 germplasm resources could be divided into 2 groups:GroupⅠmainly consisted of U.pumila germplasm,while most other Ulmus germplasm(including U.davidiana,U.lamellosa,U.parvifolia,U.macro-carpa,U.davidiana var.japonica,and U.laciniata,etc.)belonged to GroupⅡ.PCoA analysis and clustering results further verified the reliability of population division.Considering Nei's genetic distance among individuals,allele retention rate,and number of individuals comprehensively,the constructed core germplasm of 15 U.pumila individuals retained 92.4%of the al-leles from the original germplasm.The number of individuals,Shannon's information index,observed heterozygosity,expec-ted heterozygosity,and unbiased expected heterozygosity increased to 123.3%,119.3%,113.0%,113.7%,and 117.1%of the original population,respectively,indicating high representativeness.关键词
榆属/EST-SSR引物/遗传多样性/群体结构/核心种质Key words
Ulmus/EST-SSR primers/Genetic diversity/Population structure/Core germplasm分类
农业科技引用本文复制引用
代剑锋,王玉忠,徐振华,张鸿景,李亚,冯树香,郑聪慧..榆属种质资源遗传多样性分析及核心种质筛选[J].东北林业大学学报,2026,54(2):27-35,9.基金项目
河北省林草关键技术创新与示范项目(2302086) （2302086）
国家林业和草原局科技发展中心林业植物新品种与专利保护应用项目(KJZXXP202306). （KJZXXP202306）
