Abstract
Objective To investigate the influence of serum miR-135-5p and FOXN3 levels on the preg-nant outcomes of pregnant women with gestational diabetes mellitus(GDM).Methods A total of 153 preg-nant women with GDM visiting in this hospital for the regular examination and delivery from January 2023 to September 2024 were prospectively selected as the GDM group,meanwhile 112 healthy pregnant women for prenatal examination in this hospital were selected as the control group.According to whether the pregnant women with diabetes had adverse perinatal outcomes,they were divided into the good outcome group(94 ca-ses)and adverse outcome group(59 cases).The levels of serum miR-135-5p and FOXN3,lipid metabolic and glucose metabolic indicators were compared between the different groups.The Person correlation was used to analyze the correlation between miR-135-5p and FOXN3 levels with lipid and glucose metabolism indicators.The multivariate logistic regression was used to analyze the influencing factors of adverse perinatal outcomes in GDM patients.The receiver operating characteristic(ROC)curves were used to analyze the predictive value of miR-135-5p and FOXN3 levels on adverse perinatal outcomes occurrence in GDM patients.Results The weight gain value during pregnancy in the GDM group was significantly lower than that in the control group(P<0.05);the levels of serum miR-135-5p,FOXN3,TG,TC,HbA1c,FINS,FBG,HOMA-IR in the patients of the GDM group were significantly higher than those in the control group(P<0.001);while the HOMA-β level was lower than that in the control group(P<0.001);the levels of serum miR-135-5p,FOXN3,TG,TC,HbA1c,FINS,FBG and HOMA-IR in the patients with adverse outcomes group were significantly higher than those in the good outcomes group,while the HOMA-β level was significantly lower than that in the adverse outcomes group(P<0.001);serum miR-135-5p was positively correlated with FOXN3,TG,TC,HbA1c,FINS,FBG and HOMA-IR(r=0.479,0.327,0.412,0.394,0.501,0.305,0.512,P<0.05),and negatively correlated with HOMA-β(r=-0.424,P<0.05);serum FOXN3 was positively correlated with TG,TC,HbA1c,FINS,FBG,and HOMA-IR(r=0.405,0.441,0.376,0.513,0.347,0.297,P<0.05),and negatively correlated with HOMA-β(r=-0.392,P<0.05).The results of logistic regression analysis showed that miR-135-5p,FOXN3,TG,HbA1c,FINS,HOMA-IR and HOMA-β were the influencing factors of adverse per-inatal outcomes occurrence in GDM patients(P<0.05);the area under the curve(AUC)of miR-135-5p for predicting the adverse perinatal outcomes in GDM patients was 0.874,the sensitivity was 82.98%and the specificity was 72.88%;the AUC of FOXN3 for predicting the adverse perinatal outcomes occurrence in GDM patients were 0.813,the sensitivity was 77.60%and the specificity was 68.97%,respectively;the AUC of the two combination for predicting the adverse perinatal outcomes occurrence in GDM patients were 0.931,the sensitivity was 84.48%and the pecificity was 74.47%respectively.The diagnostic value of the two combina-tion was superior to that of miR-135-5p and FOXN3 alone(both P<0.05).Conclusion The levels of serum miR-135-5p and FOXN3 in pregnant women with GDM are increased.Their combination detection has high predictive value for the adverse perinatal outcomes occurrence in GDM pregnant women.关键词
妊娠期糖尿病/miR-135-5p/叉头框蛋白N3/妊娠结局/脂代谢Key words
gestational diabetes/miR-135-5p/FOXN3/perinatal outcome/lipid metabolism分类
医药卫生
