分析测试学报2026，Vol.45Issue(2)：390-397,8.DOI:10.12452/j.fxcsxb.25061401
基于网络药理学和HPLC谱效关系的黄连改善2型糖尿病潜在质量标志物研究
Potential Quality Markers in Coptidis Rhizoma for Improving Type 2 Diabetes Mellitus Based on Network Pharmacology and HPLC Spectrum-Effect Relationship
摘要
Abstract
An integrated strategy combining network pharmacology with high performance liquid chromatography(HPLC)fingerprint spectrum-effect correlation was developed to identify the key quality markers of Coptidis Rhizoma for type 2 diabetes mellitus.A compound-target-disease net-work was constructed,prioritizing six bioactive alkaloids—coptisine,jatrorrhizine,epiberberine,palmatine,obacunone and berberine—based on topological centrality.HPLC fingerprints of ten batches were established,and in vitro α-glucosidase inhibition and antioxidant assays were conduct-ed.Grey relational and bivariate correlation analyses demonstrated significant positive associations between the hypoglycemic effect and the levels of berberine,obacunone,epiberberine and copti-sine.Principal component analysis and hierarchical clustering revealed marked inter-batch composi-tional divergence.An activity-oriented quality evaluation paradigm was thus established,transcend-ing traditional compound-centric approaches and definitively designating the four aforementioned con-stituents as core quality markers for Coptidis Rhizoma in type 2 diabetes mellitus management.These findings provide a robust scientific foundation for the standardization,quality control and precision clinical application of this botanical drug,advancing the modernization of traditional Chinese medi-cine and evidence-based therapeutics.关键词
黄连/2型糖尿病/网络药理学/指纹图谱/谱效关系/质量标志物Key words
Coptidis Rhizoma/type 2 diabetes mellitus/network pharmacology/fingerprint/spectrum-effect relationship/quality markers分类
化学化工引用本文复制引用
万萧,保瑞,毛海龙,王泽峰,闫鸿丽..基于网络药理学和HPLC谱效关系的黄连改善2型糖尿病潜在质量标志物研究[J].分析测试学报,2026,45(2):390-397,8.基金项目
云南省应用基础研究-云南联合基金(202101AZ070001-036) （202101AZ070001-036）
云南省技术创新人才培养计划项目(2018HB088) （2018HB088）
云南省科技人才和平台计划(202105AG070012ZD2210) （202105AG070012ZD2210）
云南省科技人才和平台计划(202105AG070012XS23072) （202105AG070012XS23072）
云南中医药大学中药学院团队建设项目(V2023) （V2023）
