分析测试学报2026，Vol.45Issue(2)：398-405,8.DOI:10.12452/j.fxcsxb.25081902
固相支撑液液萃取/液相色谱-串联质谱法检测人血清中10种抗精神药物
Determination of Ten Antipsychotic Drugs in Human Serum by Solid-phase Supported Liquid-Liquid Extraction Coupled with Liquid Chromatography-Tandem Mass Spectrometry
刘宇凡 1赵晓婵 1张若愚 2曾永福 3张浩然 4刘凯旋 4艾连峰 5康维钧1
作者信息
- 1. 河北医科大学 公共卫生学院,河北 石家庄 050017||河北省环境与人群健康重点实验室,河北 石家庄 050017
- 2. 山东第二医科大学 公共卫生学院,山东 潍坊 261251
- 3. 成都市金牛区疾病预防控制中心,四川 成都 610000
- 4. 河北乾业生物科技有限公司,河北石家庄 050035
- 5. 石家庄海关技术中心,河北 石家庄 050051
- 折叠
摘要
Abstract
This study developed a rapid and high-throughput liquid chromatography-tandem mass spectrometry(LC-MS/MS)method for the simultaneous quantification of 10 antipsychotic drugs in hu-man serum.The sample preparation process employed solid-phase supported liquid-liquid extraction(SLE),which offers improved reproducibility and reduced matrix effects compared to traditional liq-uid-liquid extraction.Methyl tert-butyl ether(MTBE)was used as the elution solvent due to its high extraction efficiency and low residue.After extraction,the eluate was concentrated under a gentle ni-trogen blow-down process to ensure minimal loss of volatile analytes and then reconstituted with a 50％methanol-water solution to enhance compatibility with the LC mobile phase.The chromatographic conditions were meticulously optimized to enhance sensitivity and specificity for the target analytes.The method demonstrated excellent linearity over the tested concentration ranges,with correlation co-efficients(r)greater than 0.999 for all 10 antipsychotics.The limits of detection(LOD)and quantifi-cation(LOQ)were in the ranges of 0.002-0.075 ng/mL and 0.005-0.250 ng/mL,respectively,in-dicating high sensitivity capable of detecting trace levels in clinical samples.The recoveries ranged from 87.3％to 112％,confirming the efficiency and consistency of the extraction process.Both intra-day and inter-day precision,expressed as relative standard deviations(RSDs),were not more than 13％,highlighting the method's reproducibility under varied conditions.Matrix effects were evaluat-ed and found to be between 87.2％and 115％with RSD values less than 15％,demonstrating minimal impact from serum components on the accuracy of the measurements.Furthermore,the method was fully validated according to international guidelines and proven to be robust and reliable for high-throughput applications.This method offers a practical,sensitive,and reproducible approach for the simultaneous determination of multiple antipsychotic drugs in serum,supporting therapeutic drug monitoring(TDM)and clinical research in psychiatric treatment.Its high throughput capability,min-imal matrix interference,and reliability at low concentrations make it an attractive and modern alter-native to existing analytical methods.关键词
固相支撑液液萃取/抗精神药物/液相色谱-串联质谱法/血清Key words
solid-phase supported liquid-liquid extraction/antipsychotic drugs/LC-MS/MS/se-rum分类
化学化工引用本文复制引用
刘宇凡,赵晓婵,张若愚,曾永福,张浩然,刘凯旋,艾连峰,康维钧..固相支撑液液萃取/液相色谱-串联质谱法检测人血清中10种抗精神药物[J].分析测试学报,2026,45(2):398-405,8.
